Newbies setting up R: ✔️install R ✔️install Rstudio ✔️install.packages() ✔️get typing Newbies setting up Python: ✔️install anaconda ✔️stand in a pentagram ✔️call out the name of a demon overlord on a full moon with the blood of a newborn calf ❌ModuleNotFoundError

Our latest preprint and software "NanoSplicer: Accurate identification of splice junctions using Oxford Nanopore sequencing" led by Yupei You in collaboration with Mike Clark biorxiv.org/content/10.110… github.com/shimlab/NanoSp… Oxford Nanopore 1/3

Pro Tip: Rewrite, rewrite, rewrite.

Long-read scRNA-seq is awesome for studying RNA isoforms. But Oxford Nanopore has relied on matched short-reads to identify cell barcodes and assign reads to cells. Pleased to share BLAZE, a new tool to identify cell barcodes from nanopore scRNA-seq. Short-reads not required. 1/4