Hyperosmotic stress alters the RNA polymerase II interactome and induces readthrough transcription despite widespread transcriptional repression dlvr.it/RsCBfg

Now online: Shortening the #CTD of Pol II impairs enhancer transcription and gene activation: embopress.org/doi/full/10.15… This supports a model that CTD is important for Pol II recruitment to active genes by condensation: nature.com/articles/s4159… Grateful to @DarzacqClaire Xavier Darzacq

Just out in NatureStructMolBiol: Our latest paper by the Müller lab EMBL Heidelberg describing the cryo-EM structures of human RNA polymerase III in different functional states! nature.com/articles/s4159…

For the first time, researchers have revealed the atomic structure of the human minor spliceosome, according to a new study in Science. The findings provide a framework for a mechanistic understanding of the function of this ribonucleoprotein complex. ($) fcld.ly/xgmy6w2

I am happy to share our structure of the human SAGA coactivator complex, revealing the incorporation of U2 splicing subunits and a distinct architecture from its yeast counterpart. #Transcription #Chromatin #Splicing #MolecularMachines #LargeAssemblies biorxiv.org/content/10.110…

Really excited to finally have our review out on how RNA polymerase and remodelers might collaborate to restructure the genome! sciencedirect.com/science/articl…

Our SET8-nucleosome cryo-EM structures are finally published in Life Science Alliance! The structures reveal how SET8 recognizes and binds the nucleosome substrate to induce efficient mono-methylation of H4K20. life-science-alliance.org/content/4/4/e2…

If you wish to detect and image reactive nitrogen species in live cells, try our newly engineered fluorescent probe for peroxynitrite. ~110-fold turn-on response, high selectivity, and validated in macrophages and primary glial cells. Now Cell Chemical Biology authors.elsevier.com/a/1cZwK8jWWJlu…

In 1988, Paul Sigler suggested the CTD binds transcription activators: nature.com/articles/33321… Now we see that CTD shortening delays transcription activation... embopress.org/doi/full/10.15… ...and this may be due to CTD-dependent Pol II clustering at genes: nature.com/articles/s4159…

”conformation" not "confirmation"😳

Today we report in Science Magazine the development of a continuous evolution system that can reprogram a family of clinically used proteases to selectively cleave protein targets of our choosing. 1/10 science.sciencemag.org/content/371/65… drive.google.com/file/d/1PqaQSt…

TT‐seq maps thousands of eRNA-producing enhancers in human cancer cell lines, providing ~40,000 putative enhancer-promoter pairs, many at cancer‐associated genes.

So pleased we published Pycro-Manager, a match made in heaven between Python and µManager. A big step forward for open-source microscopy control software!!

New reading material arrived for the members of my lab today. Can't wait to start reading this Doudna Lab. 🙂 UC Berkeley MCB UC Berkeley @Cal

Our publication on the structure and function of the chromatin remodeler Chd1 and the histone chaperone FACT in the context of RNA polymerase II-nucleosome complexes is now online at NatureStructMolBiol! Here's the link: rdcu.be/ciybP

Approval today of what I hope will be the first of many drugs for patients whose tumors harbor mutant RAS. fda.gov/news-events/pr…

Congratulations to our chief scientific officer, Uma Sinha, Ph.D., for being named to the PharmaVoice 100 list! We admire and celebrate Dr. Sinha’s dedication to her work and patients. Read her story here: bit.ly/2TSIZ0O

If you are interested in an accessible, lay-terms summary of gene editing and associated technologies and societal issues, you might enjoy this 34-minute documentary hosted by Pietro Boselli. youtube.com/watch?v=vrncqW…

Want to understand how does Pfizer's COVID-19 Pill work? Check out my latest video on YouTube. Please SUBSCRIBE and LIKE if you find it helpful!! The Science Behind Pfizer’s Oral COVID-19 Pill (Paxlovid) – HOW DOES IT ... youtu.be/5LDACUxw4fM via YouTube

How Does Merck’s Antiviral Pill Molnupiravir Work to Fight COVID-19? Mol... youtu.be/qF9eHCQFjec via YouTube