Today in the lab: Our scientists are extracting RNA from iPSC derived myotubes

#biotech #RNAextraction #LJL #InTheLab #labwork #scientist #lajollalabs

Took my first high time resolution videos of the myotubes and they are straight flexing on me 💪

Some day soon I'll be able to join #FluorescenceFriday ...to be continued.

Very cool study

'Treatment with β-alanine, a carnosine precursor, enhanced endogenous carnosine production and decreased ubiquitin-linked protein degradation in LLC-CM treated myotubes.'

Murf1 alters myosin replacement rates in cultured myotubes in a myosin isoform-dependent manner
link.springer.com/article/10.100…

Fusion of Wild-Type Mesoangioblasts with Myotubes of mtDNA Mutation Carriers Leads to a Proportional Reduction in mtDNA Mutation Load dlvr.it/SjH08R

The Inhibition of Metabolic Inflammation by EPA Is Associated with Enhanced #Mitochondrial Fusion and Insulin Signaling in Human Primary Myotubes;

sciencedirect.com/science/articl…

The striated myotubes (red) with multiple cell nuclei (blue) characteristic of mature muscle fibers.

Credits: Kevin Murach, Charlotte Peterson, and John McCarthy

#MedTwitter #neuroscience

I promised myself that I would not work over the weekends. But, this is the reason why I came here 👇🏻♥️. Beautiful no? #myotubes #myotwitter #science

SESN1 is a FOXO3 effector that counteracts human skeletal muscle ageing

'Administration of recombinant SESN1 protein attenuated senescence of human myotubes in vitro and facilitated muscle regeneration in vivo.'

onlinelibrary.wiley.com/doi/10.1111/cp…

The dose–response effects of arachidonic acid on primary human skeletal myoblasts and myotubes

From Brandon Roberts, PhD, MBA, CSCS, TSAC-F, CISSN et al

'high concentrations of AA inhibit myoblast proliferation, myotube fusion, and myotube hypertrophy'

doi.org/10.1080/155027…

HDL subclasses enhance fatty acid oxidation in human myotubes but improve anaerobic metabolism in mouse myotubes, support the role of HDL as a circulating modulator of energy metabolism.

Finally! Our manuscript on the cancer-like metabolic reprogramming of muscle fibres has finally been accepted. A key finding is that myotubes incorporate 14C from glucose into protein. Thanks all! More to come soon!

This weeks contribution to #FluorescenceFriday : 11 d old human primary myotubes stained for DAPI and alpha-actinin Oscar Horwath

Congrats to Tabitha Cree, Prof John T. Price, the CMR's Craig A. Goodman and Team for having their image of FKBP25 stained myotubes selected for the front cover of the latest issue of The FEBS Journal.
Cara Timpani Emma Rybalka
Murdoch Children's Research Institute (MCRI)
Institute for Health and Sport, VU
AIMSS

ฅNya! Here's the latest 🗞 from [ Science Advances ]: ' SETDB1 modulates the TGFβ response in Duchenne muscular dystrophy myotubes' 🔗: science.org/doi/abs/10.112…

Exosomal miR206 was upregulated in the blood of muscle-specific Akt1 TG mice and in IGF1-stimulated myotubes. Exogenous supplementation of miR206 promoted angiogenic response in endothelial cells.
By Yasuhiro Izumiya
doi.org/10.1253/circj.…
#circ_j #CardioTwitter

Results, demonstrating that #HDL subclasses enhance fatty acid oxidation in human myotubes but improve anaerobic metabolism in mouse myotubes, support the role of HDL as a circulating modulator of #energy #metabolism .
sciencedirect.com/science/articl…

Very proud of doctorate candidates B.Gellhaus, M.Gsaenger and E.Rodenwaldt for their hard work on Foxo3 and its role in muscle decline:

Foxo3 Knockdown Mediates Decline of Myod1 and Myog Reducing Myoblast Conversion to Myotubes mdpi.com/2455700 #mdpicells via Cells MDPI

Check out the latest in our #ArticlesinPress , IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype (Simon Dreher et al.):

ow.ly/5U3X50R7GI8

#Myotubes #Contraction #Exercise

New in the #AJPEndoMetab March issue! Sex-specific increases in #myostatin and #SMAD3 contribute to obesity-related insulin resistance in human skeletal muscle and primary human myotubes
Gunjan Saxena et al.
ow.ly/kpt350Rk3sy