Extremely excited to share our bioRxiv preprint posted yesterday, on 'A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain'! A huge team effort from Allen Institute and collaborators, combining scRNA-seq and MERFISH. biorxiv.org/content/10.110…

‼️ Two huge spatial atlases of the whole mouse brain generated by two technologies ‼️
Q: Do you prefer Slide-seq or MERFISH?😉

👉biorxiv.org/content/10.110… (data online at braincelldata.org)
👉biorxiv.org/content/10.110… (need to know a companion preprint biorxiv.org/content/10.110…)

Reylo merfish. But to be honest, I don't really know why I drew this one. 🤔 Oh well.

WIP

#HBD Xiaowei Zhuang, Breakthrough Prize laureate who has helped create technologies like STORM & MERFISH that push past the diffraction limit of light to reveal images of proteins and other molecular actors in the cell. Learn more: bit.ly/xiaowei-zhuang

Photo Brigitte Lacombe

Extremely excited about the beta release of our brand-new Allen Brain Cell (ABC) Atlas web portal! Currently it includes an scRNA-seq dataset & a MERFISH dataset, each with ~4M cells, representing ~5200 clusters in the whole mouse brain. It is based on biorxiv.org/content/10.110….

Anthony 🌕 Listener One fish, two fish, myrfish, merfish

📢Vizgen Announces Major Patent Milestone for Spatial Genomics! 🧬✨ The US Patent Office has officially granted Vizgen, Inc., U.S. Patent No. 11,959,075, paving the way for innovation in the spatial transcriptomics realm. Learn more: hubs.ly/Q02t08-60 #MERFISH

Spatial transcriptomic analysis of intestinal inflammation in dynamic. One of the best and most detailed analyses of fibroblasts in DSS colitis.
#IBD & #MERFISH #SpatialTranscriptomics #IBD fibroblasts biorxiv.org/content/10.110…

Really nice preprint on 3D MERFISH using spinning disk from the Zhuang lab. biorxiv.org/content/10.110…

We did it! The Spatial Transcriptomic team Allen Institute successfully reached our first milestone! We reached 600 section imaged! That included 4 whole mice brains! It wasn’t easy and next week we keep going. Next goal 1000! #neuroscience #merfish

Yine zor olana çekiliyorsun Merfish , tebriksssss🥳🥳🥳🥳🥳🥳🥳🥳

O zaman ne diyoruz?

İPTAL İPTAL İPTAL 🙃

Nice correspondence 5-HT2A protein (L) and 5-HT2A mRNA (R; MERFISH)
#psychedelics
nature.com/articles/s4158…

Spatial transcriptomic imaging with MERFISH in the gut of a mouse colitis model. Very beautiful work, congrats to Vizgen cofounder Jeff Moffitt and his team! biorxiv.org/content/10.110…

Methods for imaging-based #spatialtranscriptomics (iST) incl. MERFISH, MolecularCartography, Xenium… are promising techs, but it feels like there is no discussion about their limitations and QC metrics. As this has been bothering me for quite some time now, I´d like to start👇

What a phenomenal meeting with an amazing group of speakers and attendees and top notch program in single-cell technologies! Thankful for the opportunity to present this work with Sam Xiaofei (Sophia) Song Jeffrey Chuang Moffitt Cancer Center The Jackson Laboratory Cancer Center at #SCT24 ! Fusion Conferences #MERFISH 😊

I took a break to watch something besides merfish but I am back to my original nonsense! 1975 version now

I'm glad last piece of my PhD work came out! What could MERFISH derived technologies could accomplish beyond this? Please follow my future work!

Excited to share my co-first author work from the ⁦Roni Nowarski⁩ and ⁦Moffitt Laboratory⁩ at ⁦⁦Harvard Medical School⁩ mapping the cellular biogeography in the #gut #colon by #MERFISH #spatialtranscriptomics and reveal fibroblast trajectories in #colitis ! biorxiv.org/content/10.110…

#SpatialTranscriptomics Developing Human Heart
12-13 pcw

scRNAseq
#MERFISH 238 cell type markers (NS-Forest2 classifier); 365 transcripts/cell

Analyzing the Cellular Communities
'within a 150 μm zone, which represented a typical diffusion distance for extracellular signalling

Molecular & Spatial Signatures of🐭Embryonic Endothelial Cells at Single-Cell Resolution
e9.5/12.5/15.5

#ArterioVenousHierarchy & #Organotypicity of Embryonic #EndothelialCell

scRNAseq
#MERFISH #SpatialTranscriptomics
#SciArt

William Pu lab Circulation Research 2024