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We used the home office to write up our #cryoem study on #anthrax toxin. Thanks to Claudia, Dennis, Christos Gatsogiannis MPI Dortmund and the Aktories group Universität Freiburg

#CryoEM Crete 2020. 19-23 October, hopefully after the Corona crisis, amazing venue and speaker/instructor lineup, lots of hands-on processing, SPA, cryoEM, , modelling and in-depth lectures. Registration is open. Deadline 30 June. Check cryoemcrete.com Please RT 1/2

crYOLO 1.7 is out! That's new: 👇

TranSPHIRE - automated SPA processing on-the-fly during image recording. Includes a machine learning-based feedback loop that adapts the workflow to any given data set (incl. filaments) live during processing. Runs on a single workstation biorxiv.org/cgi/content/sh…

Two particle-picking procedures for filamentous proteins: SPHIRE -crYOLO filament mode and SPHIRE-STRIPER - Thorsten Wagner et al. published by Stefan Raunser lab #cryoEM #DeepLearning in Structural Biology journals.iucr.org/d/issues/2020/…

With heavy heart we had to postpone our #cryoEM symposium/workshop until next year. Save the new dates: 23-27 April.2021, Heraklion Crete Registration deadline: 30. Dec. 2020 More details here: cryoemcrete.com

Successfully got #cryolo to pick some pretty challenging amyloid fibril cryo-EM images about as good or better than a human! Should save weeks of time manually picking fibrils.

Cinderella 0.7 is out! New masking and with the help of @azazellochg I fixed several issues that occur in combination with Relion classes that lead to failed training. 2,300 Relion classes provided by @azazellochg were added to the general model. sphire.mpg.de/wiki/doku.php?…

GPU ISAC v2.3 it out! This milestone release provides a GPU-powered version of ISAC that runs on a single machine w/o the previous stack size limitations, and provides class averages in the known ISAC quality. Find the download link and documentation here: sphire.mpg.de/wiki/doku.php?…

GPU ISAC v2.3.2 is out! This hotfix release includes a major improvement in system memory usage, as well as several smaller quality of life improvements. Please find the download and release notes here: sphire.mpg.de/wiki/doku.php?…

We're happy to announce the release of TranSPHIRE! Use it to evaluate and optimize your data live at the microscope. Find the download + documentation + examples here: transphire.readthedocs.io nature.com/articles/s4146…

We are happy to invite brave people to test the beta version of crYOLO 1.8 which brings tomography support! Tutorial: cryolo.readthedocs.io/en/latest/tuto…

By picking ribosomes on 5 slices from 5 tomograms, crYOLO generalized well and picked ribosomes in new unseen tomograms.

We also applied it to viruses ( thanks @pnmshah ).

Finally, SPHIRE-crYOLO 1.8 is out and here is what is new: 1/4: It contains support for picking filaments and prominent particles in tomograms. Thanks to @TackeSebastian + Zhexin (Eric) Wang for debugging this while tracing tomograms of the sarcomere! #cryoem MPI Dortmund SPHIRE

TomoTwin official release pt 1 🥳! After some code streamlining and writing documentation we’ve released the first stable build of TomoTwin. Here’s an example of TomoTwin picking Tc toxins based on a single reference without having to be trained on the protein beforehand. 🧵1/6