Today we report in bioRxiv twin prime editing, a variant of prime editing that enables in human cells targeted large DNA deletion and replacement, as well as gene-sized integration and inversion when combined with site-specific recombinases. 1/9 biorxiv.org/content/10.110…

pn.bmj.com/content/21/6/4… A terrific review of seizure semiology, with excellent videos from Fahmida Chowdhury and colleagues. Well done Practical Neurology

Now on bioRxix, we evaluate all known human pathogenic mutations for their potential ‘treat-ability’ with antisense drugs (ASOs), based upon gene and allele. Genomic labs might want to tell patients whether a drug could help. biorxiv.org/content/10.110…

Excited to share our new work on next-gen mRNA therapeutics led by talented Abhi Aditham and Hailing Shi. We developed mRNA-oligonucleotide conjugated RNA (mocRNA) to stabilize RNA and boost protein expression in mammalian cells. pubs.acs.org/doi/10.1021/ac…

Today we report in Cell an engineered virus-like particle (eVLP) platform that mediates efficient in vivo delivery of therapeutic proteins, including base editors and Cas9 nuclease, resulting in therapeutic editing in multiple mouse organs. 1/14 drive.google.com/file/d/1GdJLA4…

MEFISTO is out in Nature Methods - a framework for dimension reduction and integration of multi-modal omics data with temporal or spatial resolution. Paper at nature.com/articles/s4159…, highlights in the thread below. (1/7)

What a day! Still can't believe I'm lucky enough to be moving on to Boston Children's for residency in child neurology Boston Children’s Child Neurology & NDD Residency. So grateful for all the friends, family, and mentors who made this possible for a kid from small town Oklahoma. Love you all!

A year ago, a non-academic friend listened to a talk I gave. I thought it went great. My friend disagreed. She said that academics are experts at making interesting stuff boring—and that we should all take a speech class. So I did. And here are 6 most useful things I learned.

You grow where you’re planted — it’s the soil that makes the difference. Forever grateful to the mentors, friends, family, and patients who grew me into a physician-scientist. Thank you for teaching me what love, community, and compassion truly mean. Stay beautiful Birmingham.

I #WearOrange because no caregiver should ever have to hear that their child is gone due to gun violence. #DoctorsForGunSafety #ProtectOurKids #EndGunViolence @TheBCRP Boston Children's Boston Medical Center AAP Peds Trainees

So wonderful to map these faces from Zoom to living bodies - absolutely loved finally meeting you all. Thank you so much for welcoming us newbies in with such warmth, grace, and kindness. Thrilled to be surrounded by such an incredible group of people!

A post-publication update - our public-facing searchable web browser (available at day-lab.shinyapps.io/ratlas/) for viewing of rat brain snRNA-seq data from brain reward structures has been updated to include count data based on the latest rat genome assembly (mRatBN7.2, aka Rn7). 1/

Today we share in Cell a new review of current in vivo delivery technologies that have enabled therapeutic in vivo gene editing—including viral vector, lipid nanoparticle (LNP), and virus-like particle (VLP) methods—and application examples. 1/2 drive.google.com/file/d/1tU6-JB…

Base editors hit the clinic nature.com/articles/d4157…

Today we share in Nature Chemical Biology our development of base editor scanning to chart DNA methyltransferase 3A sequence-function. Congrats to Nicholas Lue, Emma Garcia, Liau Lab, & thanks to @JohnDoench! We're grateful to Damon Runyon Cancer Research Foundation for invaluable support. nature.com/articles/s4158…

Getting to sign out babies by costume to the Brigham and Women's Hospital NICU day team is an absolute treat, no tricks. 100% the place to be these last few nights. cbsnews.com/boston/news/ba…

As you make your rank lists, trust that gut feeling you felt on interview day. You want to be in a program where even on hard nights the culture is to support your colleagues while having fun at work. Grateful for these two as we celebrated being done on nights! 🥳#pedsmatch2023

New preprint alert from the lab! We identified a top-down slow breathing circuit that alleviates innate fear and anxiety without altering valence. Congratulations Dr. Jinho Jhang, and co-authors (Shijia Liu, and David O'Keefe). 1/3 biorxiv.org/content/10.110…

That’s my residency lil sib Amundam Mancho, MD MPH getting recognized as a “Be Exceptional” Awardee at Boston Medical Center. What a joy it’s been to work alongside her the last few weeks, learning from her compassion, grace, warmth and remarkable clinical skills. We are so proud of you!!!