Thomas Thumberger
@_thomthum_
Developmental biologist @COSHeidelberg @WittbrodtLab chalk-talker, CRISPRer, Baseeditor & glycosylation & CDG modeler.
ID: 1932218390
http://crispr.cos.uni-heidelberg.de 03-10-2013 22:12:35
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In mouse like in fish: protect your #CRISPR donor to favor single HDR events & carefully check for multimedia insertions. crucial: don’t fool yourself with too many PCR cycles that mimic a perfect integration but is only in vitro recombination. More here: academic.oup.com/nar/advance-ar…