An overview of established methodologies for investigating: • DNA–protein interactions • Protein–protein interactions • RNA–protein interactions Widely applied in molecular biology, gene regulation, and signal transduction studies.

Low temperature releases wheat seed dormancy via the TaDof-2D–miR1832–TaP450-7A module. Whole-transcriptome sequencing, germination assays, Y1H, EMSA, dual-LUC and physiological analyses reveal regulation of α-amylase activity and ABA/GA pathways.doi.org/10.1016/j.xplc…

Back to work — let’s start the year strong! 🚀 Wishing everyone a productive year filled with successful experiments and amazing research achievements. Happy New Year from YBioHub! ✨

🧬 A novel histone H3 variant, H3.14, enables rapid transcriptional rewiring during early abiotic stress responses in Arabidopsis, balancing growth repression and stress activation. 🔗 doi.org/10.1016/j.devc…

🔬 Curious about protein interaction techniques? Learn the differences and connections between IP, Co-IP, ChIP, and RIP: ybiohub.com/%E7%94%9F%E7%8…

#Bioinformatics Learn how researchers build protein interaction network figures 👇 A practical tutorial using STRING + Cytoscape for publication-ready PPI networks. 🔗ybiohub.com/%E7%94%9F%E7%8…

This review explores how microRNAs (miRNAs) regulate plant drought responses, including ABA signaling, ROS detoxification, and root development, and highlights bioinformatics and experimental tools for miRNA target validation. 🔗 doi.org/10.3390/plants…

Researchers, check this out! 🔍 A super handy list of free biology icons and illustration tools to make your research figures look professional and shine✨ Bookmark it now—perfect for your next project! 🔗ybiohub.com/%E7%94%9F%E7%8…

A review of regulatory networks controlling plant drought responses, focusing on root sensing, signaling pathways, and transcription factors such as DREB, NAC, bZIP and AP2/ERF. Helpful for anyone studying drought-related gene regulation. 🔗 doi.org/10.1093/jxb/er…

#qPCR Learn how to design qPCR primers for a common experiment used to measure gene expression levels.📊 Step‑by‑step guide for accurate and efficient primer design: ybiohub.com/%E7%94%9F%E7%8…

🔬 10 Free Tools Every Molecular Biologist Should Bookmark Save it, share it, and make your experiments easier ybiohub.com/%E7%94%9F%E7%8…

🌱 Studying plant gene function? This article introduces a practical workflow for exploring gene regulation in plants.If interested, take a look or save for later! 🔗 ybiohub.com/%E7%94%9F%E7%8…

EsERFs regulate saponin biosynthesis via protein interactions Methods: Y1H, BiFC, docking, dual-luciferase, EMSA 👉 doi.org/10.1016/j.indc…

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Discover the detailed workflow of subcellular localization in our latest article: ybiohub.com/%E7%94%9F%E7%8… We also offer subcellular localization services – just provide your gene sequence and we handle the rest! 🧬

🧬 Unlock powerful protein interaction insights with our Yeast Two-Hybrid (Y2H) service! ✅ Access to 100+ cDNA libraries — FREE to use ✅ Reliable & efficient screening ✅ Ideal for protein-protein interaction studies ybiohub.com/%E7%94%9F%E7%8… 📩 Contact: [email protected]

Long-day vs short-day plants regulate FT genes via species-specific TFs (CO, Ehd1/HD1, E1). Day-neutral tomato uses SP5G to ignore photoperiod—showing how FT networks evolve fast and differently across species. doi.org/10.1016/j.molp…

BiFC is a common method to study protein–protein interactions. This article covers the full workflow, tips, and best practices—worth saving! We also offer cost-effective BiFC services. 👉Read more: ybiohub.com/%E7%94%9F%E7%8…

🔥 New blog up! Step‑by‑step qPCR pipetting guide 🚀 Learn how to calculate reaction volumes and prepare your master mix. Save & repost if needed. ✅ ybiohub.com/%E7%94%9F%E7%8…

🧪qPCR confusing? Use our free calculator – see reaction numbers & volumes in seconds! 👇 ybiohub.com/%E7%94%9F%E7%8… 💾 Save it • 🔁 Share with your lab #qPCR