Happy and excited to share our new bioRxiv on long-term single-molecule imaging!! 🔬💥 #Breakthrough , #ProudPI biorxiv.org/content/10.110… ZMBP SFB1101 Universität Tübingen #TRR356 Nano-🧵👇

Dear RNA Pol II, what have you done to your CTD ???? 🤣

Using live-cell single-molecule tracking for your experiments, but not sure which fluorescent label to use? Our new study might help! pubs.acs.org/doi/10.1021/ac…

We present: 'Multispectral live-cell imaging with uncompromised spatiotemporal resolution'. biorxiv.org/content/10.110… 1/16

Excited to launch our cloud-based image analysis platform today at nimbusimage.org! Analyze and interact with your data, all in the browser. Follow sign-up instructions to request an account.

Bayesian nonparametric Track (BNP-Track) simultaneously determines emitter numbers and their tracks alongside uncertainty, extending the super-resolution paradigm from static samples to single-particle tracking even in dense environments. Pressé Lab nature.com/articles/s4159…

Excited to share our new paper Nature Communications. bit.ly/3WUkxbJ Single-nucleosome imaging reveals that condensins act as molecular crosslinkers, locally constraining nucleosomes to organize mitotic chromosomes. Nucleosome-nucleosome interactions constrain whole chromosomes.

Great wish list for SMLM future by Rita Strack ! 💯 👏🏻👏🏻 #SMLMS2024

Out today from the Vandenberg and Dedecker labs! PSF splitting with the ‘Circulator’, which encodes the fluorophore emission band into the PSF, improves the information content of fluorescence microscopy and enables improved super-resolution imaging and single-particle tracking.

Thr4 phosphorylation in Pol II CTD is a "phantom mark," hidden until long after transcription ends! 👻 Our recent paper shows it's masked by nearby Ser5 phosphorylation, and we revealed it using a specific phosphatase. Read more: bit.ly/3MVNLTf #Transcription

Great Idea for Poster presentation...!!! Via Caroline O'Donnell/O'Flaherty Lab (Linkedin) #scicomm #scienceoutreach #sciencetalk #scienceposter

Interested in a fully automatic analysis pipeline to quantify and classify your 2D and 3D Single-Molecule Localization Microscopy (SMLM) data in an unbiased way? Have a look at our latest work covering ECLiPSE, developed in the @Melike_Lak lab! doi.org/10.1038/s41592… 1/11

In our new paper aided by single molecule imaging of RNA and transcription machinery we demonstrate how Polycomb Repressive Complex (PRC) represses transcription in live cells 🧵 nature.com/articles/s4155… 1/9

🌌 Dive into the world of RNA Pol II, where tiny "alien" is actually clusters of Pol II CTD in different post-translational modes, forming distinctive phase-separated droplets with unique role in transcription. Check out our new paper just published 👉 rdcu.be/dTLIX

⚠️ Tag with Caution ⚠️ New preprint led by KerstinDoerner in Maria Hondele lab with contributions from Fan Cao on our side on the effects of tagging with FPs and peptides on condensation and phase separation Summary: It’s complicated See more: doi.org/10.1101/2024.1…

Our paper NatureStructMolBiol is online! We used a combination of single-molecule tracking, two-color PALM, and genomics methods to understand how pioneer transcription factors pioneer on chromatin, revealing a "confined target search" mechanism. nature.com/articles/s4159…

Check out our latest work on Pol III Molecular Cell! We explore genomic “breadcrumbs” of Pol III activity at protein-coding genes🤯Congrats Rajendra K C and to co-authors Ruiying, Sihang, and Simon U of I School of Molecular and Cellular Biology and Duncan Smith NYU Biology authors.elsevier.com/a/1jvax3vVUPRn…

Free scientific illustrations for biologists! 😍 NIH has released a library of 500+ free scientific illustrations to create figures, presentations, and illustrations! all freely available in the public domain. Retweet and spread the message! bioart.niaid.nih.gov