Had a great week at #PVA2023 ! It's amazing to see how much #cryoEM is contributing to virology research
- almost every talk/poster showed a high-resolution structure 🤩🔬 There were also some beautiful in situ #cryoET talks on virus entry/assembly 🦠

We have a new preprint for you about our efforts to solve the structure of a small membrane protein in proteoliposomes by cryoET and subtomogram averaging.

Huge effort from Scott Chang, Patricia Dijkman & Simon Wiessing, on a really challenging target
biorxiv.org/cgi/content/sh…

Working on my very first tomograms 🤗 a looo(...)oooot to improve but so happy :)
#tuberculosis #cryoET

Excited to share our new study with the Müller and SchullerJM labs, online @nature: nature.com/articles/s4158…

This is T. kivui, an anaerobic bacterium. It uses hydrogen energy to store #CO2 . How does it do it? #CryoET revealed membrane-anchored bundles of the enzyme HDCR 🍩

1/🧵

I's no secret that there's a lot more to some songs than meets the eye. Some have hidden meanings, while others are so deeply personal to the songwriter that the average listener couldn't possibly hope to understand.

So happy to see this part of my PhD journey on biorxiv!

Only possible with the support from Benjamin Ryskeldi-Falcon - all in collaboration with Dr. Steph Fowler & the Duff Lab

If you're curious about how #napari can make your tomos look like this, take a look at our methods!

#teamtomo #cryoET

First year at BPS and so happy to share it with these super cool people (pun intended). #cryoET ❄️ #BPS2023 #WomenInSTEM

Just spent an afternoon making a filament picker in napari🔬 with Alister Burt. Mind blowing how intuitive it is to make GUI tools for 3D volume manipulation thanks to napari-threedee, magicgui and the wider plugin ecosystem. Excited to learn more! #teamtomo #cryoET

Our Common Fund Cryo-EM Center Annual Meeting has officially begun! NCCAT Info NCITU SLAC PNCC US CryoEM Service Centers NIH Common Fund #cryoET #MayThe4thBeWithYou

Beautiful #CryoET work from cellarchlab.com Biozentrum, University of Basel presented by Ricardo Righetto now @[email protected] at the CCP-EM spring symposium

I reprocessed the T. kivui 70S ribosomes (EMPIAR-11058) with #RELION -4 to get a ~7 Å map in situ 🤩

This map is now deposited EMDB - EMPIAR @EBI as EMD-16451:
ebi.ac.uk/emdb/EMD-16451

#OpenSoftwareAcceleratesScience
#TeamTomo #CryoET

We’re excited to share cryo-TomoSim (aka CTS), our #cryoET modeling and simulation software, in addition to our new preprint documenting its use in training regressive denoising and semantic segmentation U-Nets.

biorxiv.org/content/10.110…

Paralyzed groom stands up to dance with bride. Made me tear up, that was beautiful!!
Credit: Cia Sinfônica Coral E Orquestra - facebook.com/ciasinfonica/
🔸 For more inspiration and uplifting stories, please follow 👉 Epoch Inspired

Those who want to be trained in #cryoEM : Did you know you can apply for an NIH funded project-tailored training at SLAC ? I just finished my three month on-site training in high resolution #cryoET and will continue to learn remotely. Big thanks to chiulab stanford for hosting me!

Interested in #actin , #cellmigration , #cryoEM & #cryoET ?
Then take a look at the review article from Florian Fäßler & Manjunath G Javoor @Schurlab in #BiochemSocTrans Portland Press Biochemical Society.

doi.org/10.1042/BST202….

Lights on(!) coincides with our first #ultrafast #LaserPhasePlate for #cryoET paper by Daniel X. Du Cell Reports Methods #OpenAccess :

bit.ly/fitzpatricklab…

#femtosecond #laser #optics #UEM #SEM #cryoEM experiments made possible by generous support from CZI Science #ImagingTheFuture

Eric Greene nature The Sung Lab at UTHSCSA aviv meir Congrats! 🍻

The latest version of Zhi Yang Tan, Shujun Cai , Alex J Noble , Jon Chen's in situ yeast nucleosome work is out elifesciences.org/reviewed-prepr…. See their raw data, tomos, & class averages here: ebi.ac.uk/empiar/EMPIAR-…

If you're interested in simulating cryoET data (say...for training deep learning segmentation models) you should definitely stop by my labmate Carson's poster on Sunday at 1:45! 'Rapid Coarse Grained Simulation of Whole Cryo Tomograms' B501 #BPS2023

We’re happy to share our #CryoET work of native #muscle in the #mavacamten relaxed state with thin #actin & thick #myosin , #titin , #MyBPC filament.

Thanks to #teamtomo MPI Dortmund, esp Davide Tamborrini Max Planck Society in collab with Gautel lab
King's College London

biorxiv.org/content/10.110…

biorxiv.org/content/10.110…
Beautiful work from Fei's lab! #cryoET #Insitu

Check our (Debakshi Mullick,Sharon Grayer Wolf&Michael Elbaum) new Protocol from JoVE on how to set up Cryo-STEM ( #CryoEM ) with the use of #SerialEM for #CryoET . jove.com/de/t/65052

We started a project using cryo-electron tomography in oocytes - and I just though this moonscape pic of an oocyte in process was super cool #cryoET #oocytes #ScienceTwitter #repro

Happy to share our finding of #tau filaments within brain extracellular vesicles in #Alzheimers disease using #cryoET , #cryoEM and #mass_spec .

Great work by co-first authors Dr. Steph Fowler and Tiana Sophia Behr, and with Duff Lab.

Preprint➡️ biorxiv.org/content/10.110…
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