I'm SO EXCITED to share our new pre-print! 🥳 You finally got your RNA-seq data. You open RStudio, stare at the screen for 5 mins...And realize you don't even remember how to import a table. How will you analyze your data?!😱We're here to help! A 🧵👇(1/9) biorxiv.org/content/10.110…

A new study in Aging Cell from Denis Evseenko's lab USC and Steve Horvath's lab UCLA describes how a key protein, called STAT3, might turn back the clock on aging cartilage that leads to #osteoarthritis. USC Stem Cell @KeckSchool_USC stemcell.keck.usc.edu/rewind-clock-o…

Buckle up! We're in for a wild ride today. A new Nature Metabolism paper by scientists from China adds a surprising twist to the long-known FTO GWAS story. The FTO locus (16q12.2) is the first ever GWAS locus to be associated with obesity and even after 16 yrs now, scientists

👏 Check out all the ARPA-H news, including information about THOR, here! arpa-h.gov/news/

“But I learned so much from the zig zag” - Katalin Kariko on her untraditional career path

Our article is now live at Nature Cell Biology! representing a fantastic collaboration with D. Allan Drummond and squire lab. We demonstrated reversible storage of stress-induced orphan ribosomal proteins within chaperone-stirred liquid condensates. Dive in nature.com/articles/s4155…

Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR go.nature.com/3NMGcyO

Really wild and cool discovery for virus nerds: an entirely new class of viroids found by Stanford's Andrew Fire and colleagues, in a search of human oral microbiomes. They encode a new class of proteins as well: biorxiv.org/content/10.110…

New preprint! Ben Adler and I Doudna Lab, in collaboration with Emily Armbruster and @JoePogliano, unveil a mechanism for Hachiman immunity. The punchline - Hachiman is a genome integrity sensor. College of Chemistry Innovative Genomics Institute HHMI biorxiv.org/content/10.110… 🧵 1/14

We know the importance of subtle gene dosage variation, and yet the main tool of molbio is gene KO/KD. In our new preprint, Júlia Domingo introduced subtle modulations of transcription factor dosage by CRISPRi/a, and quantified responses by scRNA-seq. 1/ biorxiv.org/content/10.110…

So excited to share our preprint from the El-Samad lab on engineering multiple small molecule and cell contact-inducible systems for controlling gene expression and differentiation in mESCs! biorxiv.org/content/10.110…

All these findings are perfectly in line with theory suggesting that cooperativity of transcriptional regulators is not only crucial for cell fate decisions, but at the same time makes them extremely dosage-sensitive. #haploinsufficiency 3/3 journals.biologists.com/bio/article/11…

To the top of the reading list! Some intriguing ideas and observations on modes and tuning of epigenetic memory. biorxiv.org/content/10.110…

At long last, the in4mer paper is published! In4mer is our CRISPR/Cas12a platform, developed in collaboration with John Doench, where we show that expressing 4mer arrays of Cas12a guides turns out to be a highly efficient way to knock out genes singly or in combination.

It's new-preprint-from-the-lab day! (In my opinion the most fun + authentic day to celebrate a new publication.) Check it out: "Large-scale control over collective cell migration using light-controlled epidermal growth factor receptors" biorxiv.org/content/10.110…

Ready for another bit of obscure WTF molecular biology that has an important broader message you’ll see discussed nowhere in the science press? Of course you are! Then here goes. (Warning – long thread! But with a punchline.) It’s about this paper: /1 nature.com/articles/s4158…

Today in Nature Biotechnology, we describe first-gen click editors (CEs), which couple the advantageous properties of DNA-dependent DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise and versatile human genome editing. 🧬 nature.com/articles/s4158…

Epigenome editing technologies for discovery and medicine go.nature.com/4cXw54M

More cool stuff from Victora Lab with their LIPSTIC technology - this time applied to tumors and looking at DC:T cell interactions! biorxiv.org/content/10.110…

🚨 New preprint alert! I am excited to present a collaboration between the Doudna Lab and Zev Bryant lab, with co-first authors Noor Al-Sayyad and Kevin Wasko and me. Curious about what drives high-efficiency genome editing by Cas9? Let’s dive in!👇🧵biorxiv.org/content/10.110…