New preprint. Thorben Schramm made an E. coli CRISPR library🧫 and screened for temperature sensitive mutants. Some of them are great growth switches. biorxiv.org/cgi/content/sh…

New preprint. We found that ATP limitation makes E. coli resistant to some antibiotics because it induces expression of a transporter (PunC). This happens also in E. coli from the clinic. biorxiv.org/content/10.110…

New paper from our group. We made 15,120 E. coli mutants and screened them for temperature-sensitivity. The thermo-switches are great tools to control bacterial metabolism and growth. 👇 doi.org/10.15252/msb.2…

Save the date! March 21-23 next year, please join me and Nassos Typas Typas Lab and Ruth Ley @RuthLeyMicro for this big microbiome conference. Fabulous speaker list, keep an eye on mosbacher-kolloquium.org

Turning up the heat on essential genes🌡️ Thanks Stirling Lab for this very nice comment about our thermo-switches. AcCoA switches would indeed be a very nice thing. embopress.org/doi/full/10.15…

10/2023 Issue ➡️ embopress.org/toc/17444292/2… On the cover (by SciStories LLC): Genome-scale mapping or temperature-sensitive mutations in E. coli Link Lab Universität Tübingen

We wrote a preview about a mobile CRISPRi library from the Brown Lab. Great tool to explore the different "shades" of gene essentiality: cell.com/cell-reports-m…

Great collaboration with the Milo Lab. Not much is needed to reprogram E. coli metabolism for autotrophic growth: elifesciences.org/articles/88793

Controlling bacteria, controlling metabolism, and why this is relevant for antibiotics. The #exzellenterklaert podcast featured our research, together with my colleague @NadineZiemert. Thanks to Larissa and team and to Controlling Microbes to Fight Infections . In German: exzellent-erklaert.podigee.io/42-bakterien-k…

New preprint showing the importance of the highly conserved bacterial SPFH complex for aerobic respiration. Interesting similarity to mitochondrial prohibitins. Great work by Isa Pérez López in collaboration with Link Lab and facilities of Max Planck Institute for Terrestrial Microbiology biorxiv.org/content/10.110…

Two-stage bioprocesses could overcome many limitations in bioengineering. Check out our review about this exciting topic👇 Led by Paul Hudson. Thanks for the nice collaboration. nature.com/articles/s4422…

Don't miss the #GCSB Conference⏰!  With less than a month to go, it's time to secure your spot and explore the latest advancements in synthetic biology📅. Check out the now published poster program and get your tickets right here👇 gcsb2024.de

Our group was featured in the key to my research podcast. There is a short version and a deep dive about our research and episodes with other great scientists from Universität Tübingen, @Gh_Tabatabai, Eric Kemen. Check it out: keytomyresearch.podbean.com

Congratulations Dr. Lubrano 🥳

New paper from our group: we developed intact protein barcoding, which uses small proteins as a barcode to identify CRISPRi strains with mass spectrometry. search.app/Tb7XiPbBoj1rnJ…

Our CRISPR screen in E. coli shows that metabolic mutations influence antibiotic action. Pathway-specific bottlenecks cause resistance. Slow growth leads to tolerance. Read more 👉 doi.org/10.1038/s44320…

Great collaboration with the SourjikLab lab. Check out how isoprenoid biosynthesis, high oxygen and a membrane complex influence E. coli fitness 👇

We are searching PhD students in synthetic biology and metabolic engineering uni-tuebingen.de/universitaet/k…