Excited to share my PhD work on applying Oxford Nanopore DRS and PCS from ccRCC archival tumour samples! Thanks to Libby Snell , Naveen Vasudev and everyone involved! preprint: medrxiv.org/content/10.110… #preprint #nanopore #transcriptomics #oncology #ccRCC

Direct RNA sequencing update! We are pleased to announce that RNA004 is moving into open Early Access. RNA004 provides higher outputs and accuracy compared to the previous version RNA002. Learn more: bit.ly/40KRtVI 1/9

Thrilled to release of RNA m6A model with incredible accuracy; almost 99% per-read, per-site accuracy. This opens up so much exciting research and many more modified bases to come! #NanoporeConf

Finally out! We found that mRNAs are not uniformly distributed across the cytoplasm. mRNA architecture is the strongest determinant for localization. Subcytoplasmic location of translation controls protein output. cell.com/molecular-cell…

🧬We uncovered that the RIG-I Interferon type 1 pathway is sequence dependent. It can change our insights into antiviral defence, autoimmune disorders, and the future of IVT mRNA-based therapeutics. Explore preprint: biorxiv.org/content/10.110… #RNA #Immunity #Dioscuri IIMCB, Warsaw, Poland

SHAPE-inspired RNA structure profiling at single-cell resolution!! Read about it in the paper out today from Jiaxu Wang and Yue Wan. nature.com/articles/s4159…

I am looking for a computational biologist with a PhD in genomic science to join my research team at Oxford Nanopore initially for a 1 year contract. We are doing all sorts of exciting work on RNA and DNA sequencing tech dev, including modified base calling. shorturl.at/exDX2

See our new preprint pairing Oxford Nanopore single-cell sequencing with 10x Genomics for full-length antibody repertoire sequencing of single B cells, in collaboration with Prof. Dr. Benhur Lee, MD 🦠🧬🔬 at Icahn School of Medicine at Mount Sinai. A thread 🧵 (1/14) biorxiv.org/content/10.110…

Another year of updates for direct RNA sequencing: -20% increase in output, and longer reads -Comprehensive RNA mod detection (in all contexts) including: m6A (N6-methyladenosine) & pseudouridine. -120 million short RNA reads on a single PromethION Flow Cell #nanoporeconf

📢I'm #hiring! I have two positions open in my #RNA R&D team! I'm looking for experienced RNA biologists: one #Scientist & one #Manager level. Experience w/ Oxford Nanopore #directRNA and/or #biopharma #mRNA manufacturing AD/QC desired but not required. Details👇linkedin.com/posts/libby-sn…

At #nanoporeconf, Logan Mulroney will share how using direct RNA #nanopore sequencing you can simultaneously detect all RNA mods across the human transcriptome - without the need for additional biochemical assays. Learn more at #nanoporeconf: nanoporetech.com/about/events/c…

Published today in nature, we describe an approach for single-molecule protein reading on Oxford Nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. nature.com/articles/s4158…

Oxford Nanopore Addition of m5C and inosine modified basecallers to m6A and pseU enables expanded view of RNA biology

Updates to Direct RNA -RNA004 accuracy: 98.9% -Short-read RNA sequencing (>50 nts) -Accurately basecall four modifications: m6A, m5C, pseudouridine, and inosine. #nanoporeconf

SPECIAL ISSUE! This month Genome Research publishes a diverse collection of research and review articles in a special issue highlighting advances in long-read sequencing applications in biology and medicine. tinyurl.com/Genome-Res-34-….

Come and work with us! New 3-year postdoc position in cancer immunology and transcriptomics funded by Cancer Research UK Cancer Research UK. Collaboration with Naveen Vasudev and @TurajlicLab More info and apply: jobs.york.ac.uk/vacancy/resear… Closes 19 March! It’ll be fun! Hull York Medical School, York Biomedical Research Institute

Super excited to share our latest work on #RNA liquid biopsy via Oxford Nanopore sequencing for novel biomarker discovery and #cancer early detection! biorxiv.org/content/10.110… Thank you American Cancer Society ACS Research for supporting our research!