🎉Latest PomboLab review by Tom Sparks and Izabela Harabula 'Evolving methodologies and concepts in 4D nucleome research'. MDC-BIMSB Max Delbrück Center 4DNucleome International Nucleome Consortium Helmholtz . Free access for 50 days at: authors.elsevier.com/a/1b8DR_LjpbRg… sciencedirect.com/science/articl…

Our paper is out in Nature Communications: Dynamics of the 4D genome during in vivo lineage specification and differentiation. Great teamwork with Rob Beagrie, Jim R. Hughes and Hughes Group. See thread below. #3Dgenome #generegulation nature.com/articles/s4146…

It’s my great pleasure to announce the first paper from our group. It’s a true team effort. If you are interested in biomolecular condensates, splicing and RNA biology read the thread👇. Especially if you have worked with SRSF2 or nuclear speckles. (1/9) biorxiv.org/content/10.110…

Interested in 3D gene regulation? Our group is recruiting MDC-BIMSB Single-cell omics + 3D chromatin organization + in vivo transgenic models Experimental / computational applicants both welcomed 👇 Postdoc: mdc-berlin.de/career/jobs/po… PhD: mdc-berlin.de/career/jobs/ph… Please RT🙏

Revisiting nuclear speckle proteomics with TSA-MS-Ratio: ~70% of top 100 proteins were previously shown by microscopy to be concentrated in speckles. Special focus on MFAP1, whose levels modulate speckle size and stability. doi.org/10.1083/jcb.20…

Preprint alert from PayerLab. Ever wondered how Compartments, TADs and Xist play together to switch on an entire chromosome? Then read the story by our fantastic Moritz Bauer in wonderful collaboration with Guillaume Filion and Quique Vidal. A thread. 1/5 biorxiv.org/content/10.110…

Joseph Dopie, Andrew Belmont et al apply TSA-MS ratio to identify nuclear speckle proteins and show that MFAP1 levels modulate nuclear speckle size and that MFAP1 is recruited early to reforming nuclear speckles after mitosis bit.ly/38gxR0m

Ok. I just don’t know what’s going on anymore. biorxiv.org/content/10.110…

Check out our latest work of DNA seqFISH+ now published @nature nature.com/articles/s4158…

Our state-of-the-art review on #NuclearSpeckles is out now The FEBS Journal. Many thanks to the editors and the reviewers! Reviewers were very helpful and they had great suggestions. Enjoy reading: febs.onlinelibrary.wiley.com/doi/10.1111/fe…

We are excited to share our recent work uncovering specialized genome structures in the brain, online today in nature: rdcu.be/cBvwz Prof Ana Pombo MDC-BIMSB Max Delbrück Center Helmholtz 4DNucleome NeuroCure Cluster of Excellence ↓ Short thread below ↓ 1/7

Our paper is out in nature! Extrachromosomal DNA molecules huddle together in cancer cells to activate oncogene expression (which we call ecDNA hubs). A great collaboration with Katie Yost, Howard Chang, Frank Xie lab, Zhe Liu, and others. (1/10) nature.com/articles/s4158…

biorxiv.org/content/10.110…

Thrilled to share our work on the mapping of RNA content of nuclear domains by APEX-seq! “Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns” is out Molecular Cell sciencedirect.com/science/articl… (1/8)

Finally out!!! FISHing DNA without destroying chromatin ultrastructure! Check out our new RASER-FISH protocol, conceived by jill brown and Veronica Buckle, and tell us what you think! nature.com/articles/s4159…

To advance equality for women, use the evidence nature.com/articles/d4158…

Our latest study identifies PRC2 as a differentiation gatekeeper of naïve human stem cells. Great collaboration with ⁦@simonelsasser⁩ spearheaded by ⁦Nerges Winblad⁩ ⁦Banu Kumar⁩ Carmen Navarro & ⁦John Paul (JP) Schell⁩ ⁦ nature.com/articles/s4155…

Our paper is finally out! After 10 years of model building we generate complete embryoids from three types of stem cells - they develop heart, brain structures and more. We show the first proof of concept knocking out a gene required for neurodevelopment nature.com/articles/s4158…

Excited to share my first preLight article! 🎉 Check it out and dive into the fascinating world of genome organization 🧬