So excited to share one of the final works from my PhD in Seth Shipman’s lab with Asim Khan and oso, where we used high-throughput libraries and machine learning to create design rules for retron gene editors: biorxiv.org/content/10.110…

Thank you Nature Methods Madhura Mukhopadhyay for highlighting our research: Development of Multiplexed Orthogonal Base Editor (MOBE) systems! 🧬Komor Lab UCSD Chem Biochem UC San Diego School of Physical Sciences nature.com/articles/s4159…

Check out this great summary of our work "MOBE: A Base Editor That Multitasks without Mix-ups" by The Scientist Aparna Nathan featuring Krishanu Saha Komor Lab 🧑‍🔬🧪UCSD Chem Biochem UC San Diego School of Physical Sciences the-scientist.com/mobe-a-base-ed…

Research Highlight by Madhura Mukhopadhyay: Multiplexed Orthogonal Base Editor (MOBE) systems developed by @komorlab, @quinn_cowan_sci & colleagues is a suite of tools that use RNA aptamer systems to enable simultaneous distinct point mutations at different loci. nature.com/articles/s4159…

Excited to share 'frugal' CRISPRkit, a gene editing tool for K-12 education! Developed almost entirely by high school and undergrad students, led by Marvin & Matthew, this kit brings hands-on CRISPR experiments to classrooms to explore genome engineering without equipment. (1/2)

Having a hard time with #CRISPR HDR? Your cells might be fighting against you! TREX1 nuclease, high in some cells, eats up HDR donor templates. Check out our paper in Nature Biotechnology for the story & ways to improve HDR. nature.com/articles/s4158… With Erman KARASU & John Maciejowski

Check out our latest study on the DNA polymerase theta (Polθ), a precision oncology target for HDR-deficient cancers. Our work uncovers the inhibitory mechanism of a potent Polθ inhibitor using cryoEM, accelerating rational drug development strategies: rdcu.be/dQXqB

Genome editing with DNA-dependent polymerases #NBTNV go.nature.com/3WUmCod

Saisha loves chess and singing. She is also affected by Charcot-Marie-Tooth disease, a genetic neuropathy that causes progressive damage to nerves in her legs and arms. In 2023, Saisha and her parents visited Gladstone. Read more: bit.ly/4gyGOF8

Our latest paper is out! In collaboration with Anne Carpenter, PhD, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/ cell.com/cell/fulltext/…

*Efficient PAMless base editing in patient-derived HSPCs* 🧬🖌️ science.org/doi/10.1126/sc… For a few years now, we've been collaborating with clinical experts to develop genome editing-based treatments for genetic diseases. It has been a great fortune to work with, and learn from,

So you want to engineer your hiPSCs, but targeting DNA payloads requires multiple slow, inefficient steps for each construct. What if we could accomplish multi-site integration seamlessly? 🚨Introducing STRAIGHT-IN Dual! 🧵 (1/n) Link: biorxiv.org/content/10.110…

Attention gene editors! We are searching for faculty in gene editing (broadly defined) at UC Irvine: recruit.ap.uci.edu/JPF09319

Excited to share our new system for evolving eVLPs with improved production and protein delivery efficiencies! Grateful to David R. Liu and liugroup for support throughout this study, and special thanks to co-authors Meirui An and Paul Chen for their contributions!

Today we report Evo, a generalist foundation model for biology, on the cover of Science Magazine. Trained on billions of DNA nucleotides, Evo was designed to capture two key aspects of biology: the multi-modality of the central dogma and the multi-scale nature of evolution.

Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations go.nature.com/3D2e4Fh

Circuit not working? Time to check your syntax... #synbio MIT ChemE Dept MIT Dept of BE

The Conklin Lab is hiring! We are seeking a Research Associate I to support ongoing projects focused on DNA repair and genome editing, particularly using motor neurons as a model system. Apply Here: gladstone.wd5.myworkdayjobs.com/careers/job/Sa…

1/10 Today in Science Magazine in collaboration with liugroup we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. drive.google.com/file/d/1I-UbCR…

Check out MitoScribe, our new preprint led by Linhan Wang: biorxiv.org/content/10.1... It's an analog molecular recorder that uses neutral base edits to the mitochondrial genome to store info about historical signaling in a cell. Single cell resolution at scale (see next post)!