Ever wondered how much it costs to run an early-stage biotech start-up? Or how much money you need to raise? We surveyed a dozen founder-led biotechs to find out.🧬 Dig into the Pillar VC Founder's Guide on Data-Driven Budgeting in Biotech 👇 pillar.vc/playlist/artic…

Excited to share our new study with the Müller and SchullerJM labs, online @nature: nature.com/articles/s4158… This is T. kivui, an anaerobic bacterium. It uses hydrogen energy to store #CO2. How does it do it? #CryoET revealed membrane-anchored bundles of the enzyme HDCR 🍩 1/🧵

Still very much in disbelief that two degreeless heathens in a home lab with no formal training managed to produce a reference quality bacterial genome of a really hard-to-crack microbe and that our assembly is now THE representative genome of that organism. Amateur biology FTW!

Check out Rachael Chanin, Ami Bhatt, and I's new mini-review on genomic structural variation in bacteria and how its often overlooked! From genome structure to function: insights into structural variation ... sciencedirect.com/science/articl…

The postdoc shortage has been a point of wide discussion recently 🧬 What is *actually* happening? Where are all of the postdocs going?

It's parasites all the way down! John Eppley Elaine Luo Steve Biller et al used Oxford Nanopore sequencing to characterize mobile genetic elements that **hijack phage capsids** to shuttle themselves between hosts in the marine microbial environment.

Have you ever wished to sequence SPECIFIC bacteria but NOT the vast background in #microbiome? Today, we introduce *mEnrich-seq*: #methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome. 4yr project led by Annabel Cao! biorxiv.org/content/10.110…🧵1/10

Asgard archaea reignited discussions about the tree of life 🦠 But do they have eukaryotic features? YES!! 🤩 Check out our new paper nature by Florian Wollweber and Jingwei Xu, in an exciting collaboration with the Schleper lab Schleper Lab Vienna. nature.com/articles/s4158… 🧵1/4

We are pleased to announce a new protocol explaining how to recover a DNA library mid-sequencing run. This protocol has been developed to enable precious samples to be recovered and transferred to a new flow cell for continued sequencing. Learn more: bit.ly/3QwCTfi

Great occasion for my 1st tweet! Proud contributor to this nature perspective on the ongoing debate questioning a fetal #microbiome. With 46 experts in #microbiome #science and related disciplines, we assess the evidence. The answer, an unambiguous ‘No’ bit.ly/3XF4ePg

Very proud to share that my first-author paper refuting the existence of a human blood microbiome is now out in Nature Microbiology: nature.com/articles/s4156… Huge congratulations to all co-authors and special mention to Niranjan Nagarajan and Minghao Chia. See quoted for a summary🧵.

Looking for a point + click platform for analyzing sequencing data? CZ ID now supports metagenomic analysis with Oxford Nanopore ✳️ Upload Nanopore sequencing data ✳️ No coding required ✳️ Interactive reports ✳️ Download reports Learn more about the pipeline bit.ly/42TCIQX

Come talk 🦠💻🧬 with me at #ASMicrobe! I'll be: 1. At my poster: adaptive sampling, MAGs, and host methylome from human microbiomes (Sat, HMB06) 2. Intro-ing metagenomics pipelines for Nanopore data (Sat/Sun, Data for Breakfast, 10:05-10:20 @ booth 2207) 3. At the Nanopore booth

tRNA abundance and chemical modifications are measured simultaneously with nanopores go.nature.com/3ZH3eu8

Today we report that removing a strain from a complex microbiome can ripple through the community in an unpredictable (& useful) way. Min Wang was the mastermind, with help from Mark Brown, Stan Hazen & crew. Cell 1/25 cell.com/cell/fulltext/…

In highly methylated Borg genomes, alteration of Nanopore signal by mods presented challenges but was very much a feature, not a bug 😁 Read below for many more Borg insights from @MSchoelmerich! A fun collab between my postdoc lab and my group Oxford Nanopore, esp Lynn

The prokaryotic methylation tool we all needed from @acritschristoph. Unlike Nanodisco or Snapper, it is compatible with R10 chemistry and requires only native DNA - no amplification!

#Nanopore sequencing is the gold standard for detecting base mods in DNA. Models available: for 6mA, 5mC and 5hmC. Models for 4mC (5mC/4mC) along with tools to detect DNA damage (eg: 8oxoG & RiboG) in development - only possible with #nanopore technology. #nanoporeconf

It hasn't been long since I last quantified Oxford Nanopore accuracy, but the release of Dorado v0.5.0 demanded another test: rrwick.github.io/2023/12/18/ont… Biggest improvement I've seen in a while! Most of these ONT-only bacterial genomes are now >Q60 😲