Big statement coming up: This technology will, in the next decade, supersede both lentivirus and editing+AAV. This superb method will become the one of choice to add genes to primary human cells for ex vivo engineering. I am serious on this and the statement is data-based.

I am very grateful for this fantastic paper and this incredible opportunity to collaborate with so many amazing authors. One more step for #CARTcells #mmsm and patients! Thanks Marson Lab Brian Shy Vivasvan Vykunta Justin Eyquem Tom Martin UCSF School of Medicine Gladstone Institutes Tweetorial here: 👇

Combinations of single-stranded DNA repair templates and small molecules markedly enhance genome editing go.nature.com/3dS9NYC

Authored by Andrew G. Soerens et al, we show that #Tcells are capable of proliferating in response to iterative stimulation for over 10yrs! And produce enough progeny to occupy a volume 30,000x the Earth's volume! nature nature.com/articles/s4158…

Molecular basis for potent B cell responses to antigen displayed on particles of viral size biorxiv.org/cgi/content/sh… #biorxiv_immuno

Excited to share our new collaborative work with Ross Wilson & Justin Eyquem out now in Nature Biomedical Engineering - Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes - led by dana foss, Joe Muldoon, & David Nguyen rdcu.be/daIBw (OA link)

Great to see this published and thankful to have played a small part in this story during my time with Marson Lab! Enjoyed working with my previous roommate, Dan, to develop ideas together -- Excited to see how this new tech impacts the genome engineering space!!

I am so glad someone finally published on the mechanism by which high valency antigens induce better B cell responses than soluble ones rdcu.be/dlvFO

Zikherman and colleagues describe a new mechanism by which B cells recognize virus-like antigen display as a stand-alone danger signal that does not rely exclusively on avidity and BCR cross-linking. Read it here: rdcu.be/dlGBQ nature.com/articles/s4159…

Sister’s doing a lot of big science on the other side of campus! 👏🏽

Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection ift.tt/cMkL5pG #biorxiv_synbio

🚨 New study for the lab! We developed SEED selection, a method to purify KnockIns at multiple loci in a single step. Better, It takes no space in the donor template, can be easily multiplexed and its all through negative selection! 👏👏 Chris Chang biorxiv.org/content/10.110…

Intestinal IgA production depends on GPR35-expressing cDC2 positioning guided by mast cells within Peyer’s patches De Giovanni Lab jason cyster Science Immunology science.org/doi/10.1126/sc…

First paper from my time in UCSF MSTP is out today Science Immunology! Had a blast learning from and working with De Giovanni Lab and the whole Cyster Lab UC San Francisco -- looking forward to seeing how the De Giovanni Lab continues this work!

New research in mice indicates that #MastCells secrete an essential metabolite in Peyer’s patches needed for the production of for intestinal #immunoglobulin A. scim.ag/65C #IgA De Giovanni Lab UC San Francisco

Excited to share our work developing Chimeric Antigen Receptor (CAR) Macrophages to target and resorb amyloid plaques in a mouse model of Alzheimer’s Disease! (1/4) --- Test Account Wash U Radiation Oncology WashU Medicine Department of Neuroscience WashU Pathology & Immunology WUSTL Immunology Carl DeSelm MD PhD

Excited to share this new piece! From taking art classes together back in grade school and visiting colleges together in high school to now being co-first authors, I'm looking forward to continue working and learning with Rishab Ramapriyan in our future training and careers!