New preprint alert! 📢🥳 Using multiomic profiling of #SingleCell data we integrated and analysed ~180k cells from first trimester human yolk sac (YS) Our investigations recovered cells from the contents, membrane & vitelline duct [see vid below] Here's what we learnt [1/9]👇🏾

Probing the haem compartment further, we detail haematopoietic stem progenitor cell (HSPC) emergence from hemogenic endothelium, also observed in public yolk sac iPSC scRNA-seq (Clara Alsinet Armengol et al rdcu.be/cTfOt) similar to aorto-gonado-mesenephros (AGM) [3/9]

Our data are consistent with definitive HSPC predominance over primitive by 8 PCW (Vincenzo Calvanese et al rdcu.be/cTgFF) We find differential linage potentials before and after AGM contributions to haematopoiesis in YS [4/9]

Led by RachelBotting Issac Emmanuel Goh 🇺🇦 👏🏾 An amazing effort from this duo! Give them a follow Co-authored by Antony Rose Dr Simone Webb Yorick Gitton, Human in 3D @emilyxsx Mariana Quiroga Londoño Michael Mather Dave Horsfall Nana-Jane Kwasi Clara Alsinet Armengol Bayanne Olabi 🔴 Chloë Admane [8/9]

New in @Science! 🎊 Our #Developmental Human Cell Atlas study (bit.ly/yolk_sac) uses #SingleCell profiling to map the human yolk sac, pivotal for embryonic survival & first prenatal blood and immune cells. Data-portal: bit.ly/YSportal 👈 [1/12] 🎥 by Megumi Inoue

We detail haematopoietic stem progenitor (HSPC) emergence from hemogenic endothelium, also observed in iPSC scRNA-seq data (Clara Alsinet Armengol et al) similar to aorta-gonad-mesonephros (AGM) [3/12]

We find distinct waves of HSPC (red👇) lineage potential for different blood/immune cells before/after AGM contributions to haematopoiesis in YS. Our data are consistent with definitive HSPC predominance over primitive by 8 post conception weeks (Vincenzo Calvanese et al) [4/12]

We observe a pre-AGM accelerated pathway to macrophage differentiation in the YS prior to monocyte emergence (also in iPSC scRNA-seq Clara Alsinet Armengol et al) Macrophages in the YS post-AGM are from proliferating macrophage pool and monocyte differentiation [5/12]

We also identify pre-specified TREM2+P2RY12+ microglia-like cells in the YS (transcriptionally enriched in brain microglia signature) Microglia-like cells have also been observed in pre-natal gonads and skin (@emmamarydann et al & @roserventotomo et al) 📷 by Liz Tuck [6/12]

Our manuscript was just published in Science! Blessed to have co-led with the brilliant RachelBotting, working with friends and collabs at Haniffa Lab, Wellcome Sanger Institute Cambridge University! Read about how we uncover the first emergence of blood/immune cells in human embryonic life!

IMO this must be done in all comp bio papers. Without annotations, many model explanations in comp bio papers are very hard to follow. When explaining many inputs in the likelihood or many regularisation terms - you need to label what they mean. Easier to read -> more citations.

What a brilliant way to communicate core-concepts of your equations!

Fantastic study revealing several new functions of the human yolk sac during development, including monocyte-independent production of macrophages! Congratulations Issac Emmanuel Goh 🇺🇦 RachelBotting Muzlifah Haniffa an co-authors 👏👏👏