Great to close off #ESCMIDGlobal in the last poster session with our case report from a 613 day persistent SARS-CoV-2 infection. Emma Birnie Joost Wiersinga Magda Vergouwe Marcel Jonges Dave de Leeuw, MD PhD

An immunocompromised man, who was chronically infected with BA.1.17 for more then 1,5 years, until unfortunately he died because of his underlying disease, was tested repeatly. 50 nucleotides mutated

Big thanks to this patiënt and to Magda Vergouwe, Marcel Jonges , Matthijs Welkers et al.

Our latest paper: Circulation, viral diversity and genomic rearrangement in mpox virus in the Netherlands during the 2022 outbreak and beyond - onlinelibrary.wiley.com/doi/10.1002/jm…
Marion Koopmans, publications: https://pure.eur.nl Matthijs Welkers

📄 📄 Our molnupiravir work is now out after peer-review! We definitively demonstrate that molnupiravir has resulted in viable SARS-CoV-2 viruses with significant numbers of mutations, in some cases with onwards transmission of mutated viruses. nature.com/articles/s4158…

Recently we identified a human swine flu case A(H1N1)v in a respiratory surveillance study among the general population in The Netherlands. No severe disease, and no evidence for any forward transmission.
who.int/emergencies/di…

How important is ORF9b? More important, I think, than almost anyone has realized. ORF9b is often neglected—it is ignored on phylogenetic trees & on GISAID sequences. I see 9 lines of evidence indicating it's a major player. But they require some background. /1

SARS-CoV-2 chronic infections.

Another deep dive.
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Matthijs Welkers Marion Koopmans, publications: https://pure.eur.nl Richard Molenkamp ARGOSconsortium Jelle Koopsen Kwinten Sliepen Marit van Gils 👩🏻‍🔬🦠🧬🔬

Between May-September 2022, 135 MSM were positive for #Mpox in Amsterdam. We found that anal and lesional samples🧫 had a higher viral load than throat samples. 👉Showing that in this outbreak, sexual contact was the main transmission route.
onlinelibrary.wiley.com/doi/10.1111/jd…

Among the 401 samples were

🔸2 positive #mpox cases , sampled prior to the first known confirmed case in the 🇳🇱
🔸399 negative samples, dating as far back as February 2022

= suggesting that mpox transmission did not occur before May 2022

bit.ly/EUS2817Mpox

So nice to be back at conferences again! Good representation of Amsterdam UMC by Sébastien Matamoros and GGD Amsterdam by Jelle Koopsen. #ABPHM23

1/ Every person who rapidly shares viral genome sequence data should be celebrated & I'm proud to have helped GISAID Initiative grow. I believe in the platform & its cause is noble. Most of all, I believe in the global community that is the true fabric of GISAID. But there's a problem.. 🧵

The first Coelho lab paper is out!
medrxiv.org/cgi/content/sh…
During the 2022 mpox outbreak, JYNNEOS vaccine was given to adults in the US to help prevent mpox (1/8).

Wrote 800 words on the current H5N1 situation in wildlife and the uncertainty around whether it might cause a pandemic for the Imperial Medicine blog
blogs.imperial.ac.uk/imperial-medic…

Following the death of an 11-year-old girl from highly pathogenic avian #influenza #H5N1 in Prey Veng province, #Cambodia , Ministry of Health reports 12 more infected people, 4 of whom have begun to show symptoms. Results of diagnosis expected tomorrow.
khmertimeskh.com/501244375/afte…

Very cool to see nature cover our preprint! I agree with Sarah Otto. “Given the large-scale risks of this mutagen producing new variants faster, including variants that are immune evasive, I encourage public-health leaders to call for a global halt to [molnupiravir's] use.” 1/

De groeiende varianten gingen samen in week 4 in Nederland richting de 50%. Het is nu week 6

Amsterdam UMC , inBiome, UMCG en het Erasmus MC hadden gelukkig hun samples voor week 4 snel op GISAID Initiative staan. 🙏

Ondanks het afnemende aantal samples, samen toch nog boven de 100
1/n

📢DengueSeq: A pan-serotype whole genome amplicon sequencing protocol for dengue virus

We developed primer schemes for the four dengue virus serotypes that are compatible with amplicon-based SARS-CoV-2 sequencing protocols.

Link to protocol👇
dx.doi.org/10.17504/proto…

💥 New preprint out today

We conclusively demonstrate that mutational events caused by molnupiravir treatment can be seen in globally sequenced SARS-CoV-2 genomes, in some cases with onwards transmission.
🧵
medrxiv.org/content/10.110…

A urinary tract infection caused by #Salmonella Oranienburg derived from a pet snake 🐍 in an 18-year old woman confirms the potential zoonotic risk of acquiring reptile-associated zoonoses from pets: a case report

sciencedirect.com/science/articl…