#PaperSharing #Nature #Chemical #Biology A chemically controlled #Cas9 switch enables temporal modulation of diverse effectors Autoinhibited #ciCas9 possesses low activity, but addition of a small #molecule (A-1155463) controlled DNA binding of ciCas9 nature.com/articles/s4158…

MDPI journals have been included in the list of predatory journals. It was about time. predatoryreports.org/news/f/list-of…

#PaperSharing #Molecular #Cell Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining (#NHEJ) cell.com/molecular-cell…

It was a very nice talk by Yueh Cho about the phosphorylation of ribosomal protein, which plays a role in regulating plant translations depending on the light!!

#PaperSharing The #prolfqua #R package integrates essential steps of the #MS-based differential expression analysis workflow: quality control, data normalization, protein aggregation, statistical modeling, hypothesis testing, and sample size estimation pubs.acs.org/doi/10.1021/ac…

#PaperSharing The #prolfqua #R package integrates essential steps of the #MS-based differential expression analysis workflow: quality control, data normalization, protein aggregation, statistical modeling, hypothesis testing, and sample size estimation pubs.acs.org/doi/10.1021/ac…

#PaperSharing #Nature Programmable protein delivery with a bacterial contractile #injection system Extracellular contractile injection systems (#eCISs), are #syringe-like macromolecular complexes that inject protein into eukaryotic cells by #spike. nature.com/articles/s4158…

A brilliant talk by professor @TLowePower at #NTU about the #genome study and #T6SS in #Ralstonia. What a good experience for me to talk to professor!

Second time to hear the talk from Professor @TLowePower about deeper evolutionary analysis on #T6SS in different phylotype of #Ralstonia at #AcadSinica. So surprised that they have so many VgrG encoded in their genome!

#PaperSharing #NaturePlant Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy nature.com/articles/s4147…

#PaperSharing #NatureCommunication A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution #cdGreen2, faithfully tracks c-di-GMP in single cells and with high temporal resolution over extended imaging times nature.com/articles/s4146…

#News #Nature Powerful ‘#nanopore’ DNA #sequencing method tackles proteins too nature.com/articles/d4158…

ICPPB & Biocontrol 2024, wonderfully organized

Congrats to Xuan (Stephenson) Lai my Ph.D student for being awarded 1st place in the poster presentation @ Annual Bacteriology Conference. Glad to see the active participation of my students in this important event gathering scientists working on #bacteria in #Taiwan. #ProudPI IPMB Academia Sinica (中研院植微所)

#PaperSharing #Nature Multi-pass, single-molecule nanopore reading of long protein strands Concept of nanopore sequencing for folded end-to-end protein domain by using ClpX unfoldase and CsgG nanopore. Protein sequencing will be easier someday future. nature.com/articles/s4158…

#PaperSharing #Chemical #reviews Cell-Free Gene Expression: Methods and Applications The cell-free expression is what we need in the future, especially for expressing those toxic effectors. pubs.acs.org/doi/10.1021/ac…

#PaperSharing #NatureBiotechnology it's time to write science! Shiny title, simple and short writing without complicated thinking, just like talk to our parents. Writing, thinking, and communicating. nature.com/articles/s4158…

A new study shows a charged, folded protein can bind an #intrinsically #disordered protein (#IDP) without triggering structure formation. The charge drives specific, disordered, and tunable #interactions. #PaperSharing #NatureCommunication nature.com/articles/s4146…

Excited to share our new work mBio reporting two #Agrobacterium DGC proteins control virulence and #T6SS interbacterial competition via distinct strategies. Congrats to 1st author, my PhD student Xuan (Stephenson) Lai for the achievement and co-authors Manda Yu IPMB Academia Sinica (中研院植微所).

Quantitative dissection of #Agrobacterium T-DNA expression in single plant cells reveals density-dependent #synergy and #antagonism #PaperSharing #NaturePlant nature.com/articles/s4147…