The MemBright PM stains set a high bar for specific membrane labeling. But if you care about the phototoxicity under time-lapse and SR imaging? Here we present a gentler palette -- PK Mem dyes. Tailored for live cell SIM/STED! biorxiv.org/content/10.110…

Gorgeous STED imaging of mitochondrial cristae and mtDNA 😍😍😍 nature.com/articles/s4137…

HBmito Crimson unveils the mysterious interaction between mitochondrial cristae and mtDNA, with 39 nm STED resolution, >1.5 hour imaging, in healthy, apoptosis, and ferroptosis! medchemexpress.cn/hb-imm-d-red.h… nature.com/articles/s4137…

sCMOS is popular in fluorescence microscopy, yet its speed is limited by the exposure-readout process. Here we designed a parallel acquisition mechanism, to use galvo to project the image onto different ROIs of sCMOS, fully using the exposure-readout time. nature.com/articles/s4137…

One day in Osaka, short but memoriful!

In the journal eLight, scientists developed a new method to improve resolution and reduce noise in fluorescence microscopy images. #Super-resolution imaging #Fluorescence microscopy Details: doi.org/10.1186/s43593…

With MRA, we are aiming at providing an open-source alternative to Huygens deconvolution. 😀github.com/YiweiHou/MRA-S… elight.springeropen.com/articles/10.11…

Our latest study published in eLight introduces a physics-rooted computational method to enhance fluorescence microscopic imaging. Our MRA deconvolution algorithms improve SNR by up to 10 dB and ensure fidelity, enabling more challenging imaging tasks. bit.ly/3MnYPYQ

🔬 We introduce MC-ISM, a groundbreaking #superresolution technique based on multi-confocal ISM, with 110 nm resolution, allowing continuous imaging of mitochondria for 1000 frames and imaging depth of 175 μm. NSR_Journal doi.org/10.1093/nsr/nw…

🚀 A groundbreaking advancement in SIM! Xi group Peking University introduces a novel Fast Reconstruction-3DSIM The Innovation: a Cell Press partner journal, accelerating reconstruction speed by up to 800-folds, paving the way for high-throughput, near real-time 3D super-resolution imaging. tinyurl.com/he96a64j

A Story of Envy,Jealousy,and Regret:autonomous driving is definitely the trend of the future.Once it is fully implemented,people might not need to buy their own cars anymore,and urban traffic problems would be instantly solved.The tide of technology is unstoppable.

Our latest long review explores the principles, advanced instrumentation, and cutting-edge applications of SIM in live-cell imaging. Both SIM (stripe-based) and ISM (point-based) are included with their derivatives. #Microscopy #SuperResolution tinyurl.com/b84479hr

Watch the Story Video to the Review Article: youtube.com/watch?v=QcLt-O… #PKUResearch #InnovateWithPKU Peng Xi

For a quick summary of the paper, read the Research Briefing here: nature.com/articles/s4159…

Our groundbreaking Dark Sectioning is now published in Nature Methods! 🎉 It supercharges existing imaging systems: 🔬Widefield → Confocal 🔬 Single-photon → 2P 🔬 2D-SIM → 3D-SIM Now integrated in Airy PolarSIM and #FlexSIM! Matlab+Fiji pluin tiny.cc/wqxj001 Peking University

Congratulations to Suyi Zhong, Wei Ren, Ziyi Yang, and Hongyu Wang!

C²SD-ISM combines physical defocus rejection (spinning disk) with adaptive algorithm (DPA-PR), breaking depth limits in tissue super-resolution (↑180 μm), enhances fidelity (92% linear correlation), and supports high-throughput imaging. doi.org/10.1038/s41377… Github.

🔬 Meet 3I-SIM! Instead of stripe pattern, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 time-points, 13 h neuronal growth-cone movies, ER–lipid-droplet “track-switches” & millisecond tango. doi.org/10.1038/s41566… Nature Photonics