New work led by Victor Tieu Victor Tieu & collaboration with Dr. Crystal Mackall, in Cell Cell, for T cell transcriptome & metabolic engineering for a better cancer therapy. news.stanford.edu/2024/02/21/new…

Now online! A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells dlvr.it/T35n3V

big thanks to nature for featuring our work!!

Lead author Victor Tieu, Stanley Qi Lab, Crystal Mackall @MackallLab & team discovered a way to potentially improve CAR T therapies using a CRISPR RNA-editing platform—Multiplexed Effector Guide Arrays (MEGA)—that boosts the cells’ ability to target tumors stanford.io/49LV20X

SCI members Stanley Qi, Crystal Mackall & team pioneered MEGA, a #CRISPR RNA-editing tool enhancing #CARTcells precision against tumors. stanford.io/3TdepLd Stanley Qi Lab @MackallLab

A CRISPR-Cas13d based platform MEGA enables safer, more controllable editing of T cells to enhance CAR T therapy via reversible, multiplexed gene knockdown without cutting genomic DNA. #NBThighlight cell.com/cell/abstract/…

Check out the latest findings from PICI Center Director at Stanford Medicine Crystal Mackall, MD, @MackallLab. The PICI-funded research suggests cell therapies for #cancer could see improvements with use of a new CRISPR RNA-editing platform called MEGA. Learn more in Cell

wow!! thank you ACIR & lauren hitchings for the wonderful writeup and visual summary of our work!!

In summary, memory programming (but not TOO much memory) is a great thing for CAR T! FOXO1 is critical for mediating CAR T function and FOXO1 overexpression represents a broadly-applicable approach to enhance CAR T antitumor activity. The jury is still out on TCF1/TCF7. 13/

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thanks Genome Engineering Seminars @HMS Harvard Medical School for inviting me to give a talk on MEGA recently!! really great opportunity for young scientists/first authors to present & discuss their work. seminars are weekly, totally free to attend (zoom), and recorded/posted here: gess.hms.harvard.edu

Nature research paper: Engineered CD47 protects T cells for enhanced antitumour immunity go.nature.com/4bl7dmG

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations go.nature.com/3UTnSXM

Bidirectional epigenetic editing reveals hierarchies in gene regulation go.nature.com/4apFU9q

when in new york

yes!! i've long suspected that this was the case since essentially all prior cas13d collateral cleavage studies were done in transfected cell lines. it's so great to see this important & rigorous study out – congrats to the team!!

We’re still in our BRAT summer by sharing our new RNA resources! Improved understanding of RNA over the years has led to huge developments, such as creating a COVID vaccine that saved millions of lives. Learn more in our new fact sheet! genome.gov/about-genomics…