According to DNA methylation "aging clocks", physical activity "rejuvenates" athletes immediately after the game. 𝐔𝐩𝐨𝐧 𝐫𝐞𝐬𝐭, 𝐭𝐡𝐞 𝐫𝐞𝐣𝐮𝐯𝐞𝐧𝐚𝐭𝐢𝐨𝐧 𝐝𝐢𝐬𝐚𝐩𝐩𝐞𝐚𝐫𝐬. This is inconsistent with my definition of aging. 🧵

MOSAIC large field of view 3D single molecule localization microscopy of mitochondrial outer membranes and nuclear membranes in fixed U2OS cells.

more negative metformin and cancer data. when will this end?

Prof. Nikolai Slavov While whole-genome sequencing using Illumina is commonly described as such, in reality, it is closer to half genome sequencing due to limitations of sequencing (GC content) coverage, not to mention variant calling limitations that you allude to.

The goal of a PhD is not to learn some facts or read a few papers or learn a bunch of techniques. The goal of a PhD is to learn independence, problem solving, how to finish things you start, resilience, & gain the ability to adapt & think creatively. Learning these things is hard

Common thought is diabetes & obesity would be worse for cancer due to increased supply of glucose for the tumor. However, using a breast tumor model, diabetic obese mice have no difference in tumor glucose metabolism compared to control diet. link.springer.com/article/10.100…

A great thing about working in a lab is being involved in lab meetings and witnessing everyone's projects take shape. A single project may seem to go slow but all projects together is impressive. Caroline Bartman discussed this on the podcast: podcasts.apple.com/us/podcast/28-…

Congratulations to ⁦Max Valenstein, MD, PhD⁩ and Max Wranik and senior author Kacper Rogala on this beautiful work showing in atomic detail how amino acids activate mTORC1 via GATOR2. nature.com/articles/s4158…

We present our MetaProViz #Rpackage for #metabolomics analysis & prior knowledge integration to generate mechanistic hypotheses on how metabolic changes affect metabolite classes, pathways & environment interaction 🔗biorxiv.org/content/10.110… 📦 saezlab.github.io/MetaProViz/ 🧵 Thread

Discovery happens less when you're trying to be the expert and more when you're trying to be the learner.

Caution around isolated enzyme experiments, I love this example: SUCL activity is due to succinyl-CoA production from AKGDH. Thus you can genetically down regulate AKGDH, isolate mitochondria and test maximal SUCL activity and see no difference. pmc.ncbi.nlm.nih.gov/articles/PMC36…

And here how brain tumors exploit glucose for their growth, along with a dietary restriction of an amino acid therapy to prevent it nature.com/articles/s4158…

Successful day, love getting results that make me question the traditional 'cycle' of the TCA cycle 🤓

Slight caution: Labeling glutamine, blocking glutaminase, and *only* looking at labeled extracellular succinate might not give clear insight into the mitochondrial pathway. AKG can be exported & converted to succinate outside of the mito. nature.com/articles/s4146…

Sometimes negative data IS the data 🤨🤔

One of the country's foremost food policy experts said Tuesday that HHS Secretary Robert F. Kennedy Jr.'s Make America Healthy Again report is "a report about intentions. It's not about actions." "What you wonder is, how on earth are they going to do these things? What do they