A pioneering adventure by Matylda Izert - the first way to do protein knockdown in E.coli! Of unmodified, degronless targets😍Using just simple expression from a plasmid encoding a fusion peptide - "CLIPPER" acting as BacPROTAC. Even tough GroEL went down💪biorxiv.org/content/10.110…

Yet another paper on high resolution microED/3DED data from Uniwersytet Warszawski produced on our Glacios microscope journals.iucr.org/paper?buy=yes&…

TAAM refinement on high-resolution ED/MicroED data Structural Chemistry IUCr doi.org/10.1107/S20532… ChemistryUW Uniwersytet Warszawski Narodowe Centrum Nauki @PLGrid POLISH SCIENCE #CENT #3DED #electrondiffraction #MicroED #microcrystal #TAAM #IAM #crystallography

Another fantastic collaboration with Agata Starosta 😍We can finally play with some ribosomes too! 🤗 Frozen yesterday by Kasia, screened today by Tomasz Góral on our Glacios Uniwersytet Warszawski, looking good on the very first try!🤩

A great work on Hirshfeld atom refinement and dynamical refinement of hexagonal ice structure from ED data has just been published in #IUCrJ. All measurements were done on our Glacios microscope. doi.org/10.1107/S20522…

#cryoEM with energy filter & electron counting significantly increased resolution even for a full size protein like proteinase K. Sub-atomic resolution #MicroED rivals best reported Xray of Proteinase K but by using crystals a billionth the size. A section of the map is shown

Building on the work of Richard and Chris, here is our latest work showcasing the potential of the 100 keV #Tundra microscope with the Ceta F and Falcon C. We achieved high-resolution structures for a variety of protein complexes🧵.🔬#100keV #CryoEM. Movie by lingboyu

I'm glad to announce that we've received a nearly 1 mln PLN grant from the Polish Ministry of Science to maintain our Glacios microscope at CeNT UW. We warmly invite all interested users to take advantage of the services offered by our laboratory. cent.uw.edu.pl/en/blog/spub-g…

A new micro-ED paper from our lab has just been released highlighting the potential of electron diffraction in plant biomineralisation. #microed, #Glacios, #cryoEM mdpi.com/3001564 #mdpimolecules przez Molecules MDPI

All welcome to join our seminar summarising 5 years of cryo-EM Core Facility at CeNT UW. cent.uw.edu.pl/en/blog/semina…

After ~5.5 years our Glacios is getting a new heart... We are replacing the X-FEG emitter so we continue providing with high quality results in the future.

A brand new FEG emitter on our Glacios allowed for a significantly reduced exposure times for a given dose which for the first time resulted in an acquisition rate exceeding 300 movies/hr! (Falcon3 detector in linear mode with AFIS enabled)

Please have a look at this beautiful work of one of our past users dr Piotr Szwedziak who demonstrated that cryo-ET is doable on 200kV microscopes even without energy filter. All data shown here were collected on our Glacios. biorxiv.org/content/10.110…

Combining cryo-EM results with molecular dynamics simulations can lead to accurate determining the positioning of memebrane-active peptides within a lipid bilayer. Check out this great paper published in #JACS with data acquired on our Glacios microscope. pubs.acs.org/doi/10.1021/ja…

The article was published in JBC and is available here: jbc.org/article/S0021-… I wish to thank all the authors IIMCB, Warsaw, Poland Malopolska Centre of Biotechnology UJ SOLARIS Centre Krzysiek Wycisk Marcin Warmiński Jacek Jemielity Sebastian Glatt and all twitterless collaborators!

The latest paper in #JACS features high resolution structures of flexible MOFs that were obtained from nanocrystals and measured on our Glacios using #microED pubs.acs.org/doi/10.1021/ja…

Here it comes! A long-awaited paper led by Matylda Izert and Maria GóRNA is now out. It features multi-mode approach combining different techniques including our Glacios-made 2.45A cryo-EM stucture of GroTAC peptide interacting with GroEL protein (PDB ID: 8S32).

Another fantastic results published in #JACS. This work shows a great potential of #microED for studying mineralogical and geological samples. ED data were acquired on our Glacios at CeNT UW. pubs.acs.org/doi/full/10.10…