Day. 5 'Building' up to the Holiday Season - Day 5. Mass Spectrometry at Peak Proteins (part of Sygnature Discovery). In addition to part of our QC, we offer Mass Spectrometry as a standalone service. Find out more lnkd.in/d4GittbV

'Building' up to the Holiday Season - Day 6. Protein Expression and Purification at Peak Proteins (part of Sygnature Discovery ). At Peak Proteins we can express and purify proteins from E. coli, insect and mammalian cells. lnkd.in/d5Qa27t7

'Building' up to the Holiday Season - Day 7. At Peak Proteins (part of Sygnature Discovery ) we have extensive Protein Crystallography capabilities. This is driven by our investment in specialised crystallography robotics including SPTLabtech 's Mosquito.

'Building' up to the Holiday Season - Day 9. Antibodies at Peak Proteins (part of Sygnature Discovery ). We have supported many antibody development projects including: ➡️ Multispecifics ➡️ ADCs ➡️ T-cell engagers ➡️ Fc fusions ➡️ Antibody fragments

'Building' up to the Holiday Season - Day 10. Cell Science at Peak Proteins (part of Sygnature Discovery). At Peak Proteins we can express proteins from a number of different cell types. Last year we introduced expression from CHO cells. lnkd.in/e7QnKFJX.

'Building' up to the Holiday Season - Day 11. Expression optimisation at Peak Proteins (part of Sygnature Discovery). We recently applied Design of Experiments (Doe) towards optimising expression from our mammalian HEK293 6E cells. 📖 Read more lnkd.in/edut_ibu

'Building' up to the Holiday Season - Day 12. Cryo-EM at Peak Proteins (part of Sygnature Discovery). We are pleased to now be offering Cryo-EM alongside our other protein structure services (X-ray Crystallography and NMR).

Building' up to the Holiday Season - Day 13. We regularly produce protein for SPR. This can be for direct client use or we can perform SPR in-house - Sygnature discovery has extensive SPR capabilities and multiple Cytiva Biacore systems.

'Building' up to the Holiday Season - Day 14. NMR at Peak Proteins (part of Sygnature Discovery). We’re very excited to be offering NMR data collection and interpretation for our clients and have a number of projects underway. 🙌🏽🧲

'Building' up to the Holiday Season - Day 15. Protein Structure Determination at Peak Proteins (part of Sygnature Discovery). This structure is ATP synthase which catalyses the formation of the energy storage molecule adenosine triphosphate (ATP).

'Building' up to the Holiday Season - Day 16. We can produce high quality protein, tailor-made for SPR. As part of Sygnature Discovery we use SPR for hit identification, orthogonal hit confirmation and hit-to-lead activities. This model depicts antibody binding to an SPR chip.

'Building' up to the Holiday Season - Day 17. As part of our Cryo-EM workflow we offer the option of preparing negative stain grids & analysing them under a Transmission Electron Microscope. Here we assess the protein particle quality and suitability for proceeding to Cryo-EM.

'Building' up to the Holiday Season - Day 18. Kifunensine and X-ray crystallography 🫱🏽‍🫲🏽 A common technique we employ at Peak Proteins is to grow cells in the presence of kifunensine then treat with Endo H to enhancing crystallisation.

'Building' up to the Holiday Season - Day 19. At Peak Proteins, we leverage cloud-based computing strategies through Amazon Web Services (AWS) to enhance efficiency and scalability in our structural data processing.

'Building' up to the Holiday Season - Day 20. Synchrotrons and Structural Determination. We use Synchrotrons like the one at Diamond Light Source to solve protein structure. This model is a diorama of the i24 beamline.

'Building' up to the Holiday Season - Day 21. Protein Purification at Peak Proteins (part of Sygnature Discovery). High quality protein purification is the backbone of what we do at Peak Proteins and the workhorses of this are our Cytiva AKTAs.

'Building' up to the Holiday Season - Day 22. Peak Proteins and IniXium - stronger together Following Sygnature Discovery 's acquisition of NuChem Sciences we are delighted to combine Inixium and Peak Proteins into one Protein Science and Structural Biology division.

'Building' up to the Holiday Season - Day 23. Counting Cells at Peak Proteins with the Beckman Coulter Life Sciences Vi-CELL. Using this at Peak Proteins has allowed increased capacity in the cell expression team, and enabled more small scale optimisation experiments to be performed.

Yesterday was #InternationalLegoDay, so we wanted to share this fantastic LEGO diorama of our I24 beamline made by Peak Proteins last year! 🧱⬇ Read more about I24 here: diamond.ac.uk/Instruments/Mx…

Join #EMBOPEPC to learn how to troubleshoot in the wet lab during solubilisation and reconstitution, and how to conduct experiments at the beamlines and during early stages of cryoEM screening. 🔎⚡️ ✍️ Apply by 17 June 📍 EMBL Hamburg ➡️ s.embl.org/pep24-01 🔸 Speakers and