Our grad students deserve better, Johns Hopkins University. They could use a bonus right now. #priorities

Ariel Gershman with a great 2 minute flash talk on her work looking at epigenetics on a complete human genome #AGBT22 #T2T

At #AGBT, Sheridan Cavalier will present their team's research: 'Single-cell transcript #isoform sequencing of the actived adult mouse hippocampus with 10x #Genomics and Oxford #Nanopore'. Find out more: bit.ly/3xc4EjY #AGBT22

Long native Oxford Nanopore reads used to measure telomere length in yeast and reveals significant biological variation #AGBT22

After dinner, check out Sheridan Cavalier, PhiD 's talk in the Technology Session (7:50PM) on "Single-cell transcript isoform sequencing of the activated adult mouse hippocampus with 10x Genomics and Oxford Nanopore"

#AGBT2022 Come to the Technology Concurrent Session in the Floridian Ballroom to hear me stumble my way through explaining #SingleCell sequencing of mouse brains with #OxfordNanopore and #10XGenomics

#AGBT22 Cavalier, John Hopkins Neuroscience. Activity induced transcription produces alternative splicing at single cell in brains by 10x Genomics and Oxford Nanopore . Used R2C2, using RCA, then concatamer on ONT for alt splicing.

.Sheridan Cavalier, PhiD from Winston Timp lab: identifying and quantifying isoforms is easy using Oxford Nanopore full length RNAseq and the fragment length distribution we see on the tape station is the same as the resulting read length #AGBT22

If the key phrases "CRISPR regulation", "anti-CRISPRs", or "the existential dread of building a new model system" excite you and you're at #ASMicrobe come to my poster Friday- its HMB700:3513, "Phage-encoded Cas9 inhibitors stimulate CRISPR-Cas induction". See you there!

I couldn’t help but ‘photobomb’ this group of key contributors to the #T2T Consortium at #ABGT22, appropriately celebrating their publications reporting the first truly complete sequence of a human genome! This small group represents the larger group of true genomics heroes!

#AGBT2022 @ChrisVollmers : basically use #R2C2 for everything. It cures grandmas, it kisses babies, it solves world hunger, it makes NGS accessible

I made it to Vienna and #ESHG2022! Tomorrow I'll presenting my talk "Long-read sequencing reveals heritable large structural variants induced by CRISPR-Cas9" during the "What's new?" plenary session. Hope to see you there!

Take home messsge from Ida Hoijer #ESHG2022: Nano-OTS LRS enables detection of large CRISPR/Cas9 induced #structuralvariants that may be missed by other technologies such as short-read NGS or Sanger sequencing #zebrafish

So happy we were all binned together @binningsingletons.com on bluesky ♥️🦠

Check it out! This manuscript describes how endocytosis at synapses is coupled to exocytosis by membrane biophysics - lateral membrane compression against the actin corral does the job! biorxiv.org/content/10.110…