
SEVAplasmids
@sevaplasmids
A non-profit Open Access repository of standardised plasmids following the SEVA format. Check out our database and request your #plasmid free of charge #synbio
ID: 1150750189063417856
http://seva-plasmids.com/ 15-07-2019 12:53:19
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Researchers at Tsinghua University used #SEVAplasmids to implement a lactose-inducible T7-like system in Halomonas bluephagenesis. This system achieves higher yield than IPTG-based systems reducing cost and toxicity 👉sciencedirect.com/science/articl…


AI-designed CRISPR enzymes? Using machine learning and high-throughput engineering, researchers at Harvard Medical School created custom-made Cas9 enzymes with tailored PAM profiles. This offers higher precision and fewer off-target effects than existing tools. 🔗nature.com/articles/s4158…



A modular cloning strategy combining Golden Standard and MoClo enabled modular expression of PHB biosynthesis operons in E. coli, achieving notable polymer yields. A neat piece of work by Juan Nogales and his team. #SEVA plasmids put to great use! 🔗 academic.oup.com/nar/article/51…



New phi15-based expression system in Pseudomonas putida uses #SEVA plasmids for high-yield gene expression. The work by Nikel SEM Group shows how single-copy vectors minimise toxicity, achieve 200×induction, and outperform T7 systems. 👉 nature.com/articles/s4200…


🧬 The language of SEVA expands! The group of Pablo Iván Nikel @pabnik.bsky.social use #SEVAplasmids nomenclature in the design of CIFR—a novel iterative transposon tool for genomic engineering of Gam-negative bacteria 👉 doi.org/10.1016/j.ymbe…

🔥Bye Crabtree! At Hunan University, researchers reprogrammed S. cerevisiae to bypass the Crabtree effect, boosting lactate & 3-hydroxypropionic production by 8-11 times. A major step toward carbon-efficient biomanufacturing 👉nature.com/articles/s4146…


🔍Problems with synthetic C1 metabolism? The Pablo Iván Nikel @pabnik.bsky.social group identifies seven major barriers limiting its deployment in microorganisms and proposes specific strategies to overcome each of them. Check out their paper 👉doi.org/10.1093/femsre…

What ways to produce lignans de novo can you think of? Researchers at the Chinese Academy of Sciences made it by mimicking plant metabolism in a consortium of synthetic yeasts. Find out in detail: 👉doi.org/10.1038/s41589…