Excited to share that our manuscript exploring the analytical merits of the Orbitrap Astral mass spectrometer is out in Nature Biotechnology. Using nDIA, we profile >100 full yeast proteomes per day, or 48 human proteomes per day of ~10,000 proteins. rdcu.be/dxwEi

A dream of our lab has been to image the full central dogma from a single endogenous gene, all live and with single molecule resolution. After many years we are happy to unveil a beautiful cell line that makes it possible. Check out our preprint (biorxiv.org/content/10.110…)! (1/n)

Do you need an intro to analyzing (prote)omics data? This series of lectures may be helpful. We aimed to make them short & accessible. Enjoy watching & learning! youtube.com/playlist?list=…

Yet another must read from YingZhu (nice to see Fengchao Yu and Alexey Nesvizhskii on there too!). Impressive depth and throughput for 50µm spatial resolution. Proteome-scale tissue mapping using mass spectrometry based on label-free and multiplexed workflows biorxiv.org/content/10.110…

#TeamMassSpec We're recruiting postdocs to work on new methods to map proteome remodeling due to chemical perturbations, aging, development, and more! If you're interested, see the job posting: ap.washington.edu/ahr/position-d… and some of the lab's projects: schweppelab.org/projects

Want to learn how to optimize your #DDA-PASEF acquisition for #immunopeptidomics? Use Thunder-DDA-PASEF! See our paper by DavidGZ from HI-TRON Mainz: rdcu.be/dA7di Thanks to a wonderful collaboration with CompOmics and Christine CARAPITO!

Happy to contribute to this study. Group-specific FDR estimation is one of the strategies to control the error rates in proteogenomics searches for noncanonical peptides. There is tutorial on how to annotate the database and perform group FDR in #FragPipe: fragpipe.nesvilab.org/docs/tutorial_…

The championship round is here.. Vote #TeamMassSpec.. Let's show CRISPR how the champions do.. asbmb.org/journals-news/…

Check out figure 2 in this paper. Single cell protein quant improvements. Important advance if reproducible nature.com/articles/s4159…

T-FINDER: A highly sensitive, pan-HLA platform for functional T cell receptor and ligand discovery biorxiv.org/content/10.110…

Congratulations Fabian Coscia and team. Great work!!

New mass spectrometry techniques significantly enhance the detection of HLA class I peptide ligands, paving the way for targeted vaccine and #immunotherapy development ⚡️ #immunopeptidome doi.org/10.1038/s41467…

Very nice birthday present for Albert. The first timsOmni trap is currently being delivered to Albert’s lab.

Congratulations Doris Kaltenecker Rami @[email protected]

Friends, #FragPipe 22 has been released, and it's a big update! diaTracer enables spectrum-centric analysis of diaPASEF data. Skyline integration. Koina server for more deep-learning prediction options. DDA+ mode for ddaPASEF. DIA glycoproteomics and new chemoproteomics workflows

So excited to host Prof. Lingjun Li Lingjun Li Research Group from UW–Madison, an esteemed leader in mass spectrometry, at Yale Pharmacology!! Join us tomorrow, June 27th, at 12:30 PM in Brady Auditorium for her talk on "Advancing Biomedical Research via Innovation in MS-based Approaches." Yale School of Medicine

Two papers in nature present a new genome editing technique that enables the insertion, inversion and deletion of long DNA sequences at user-specified genome positions. The approach may provide an easier method of genome editing. go.nature.com/3RGkI8M go.nature.com/3RHBvIs

A protocol paper describing how to acquire and analyze multiplex DIA (mDIA) special proteomics data, with a tutorial on building a spectral library from ddaPASEF using #FragPipe. And now with diaTracer in FragPipe22, you can build a hybrid DDA+DIA library! link.springer.com/protocol/10.10…

Now online - check out this video from Karin Strube where Juliane Walz introduces our new phase I #ClinicalTrial for an #immunopeptidomics-based #peptide #vaccine for #patients with #AML. youtube.com/watch?v=tFuY4X…

timsTOF Compass 2025 is now available. - ICC 2.0 (Ultra 2 ONLY) - MALDI PRM-PASEF I've also included a page from the manual which explains more about ICC 2.0. It requires calibration with a high complexity sample to determine optimal ion counts.