🧬 Real-time imaging is more than speed - it’s about capturing biology in motion. RT‑SuperES enables researchers to visualise transient cell states previously missed by static snapshots. Stay tuned for dynamic data. #RTSuperES #LiveCellImaging #StemCells European Innovation Council #PathfinderOpen

Saturday Delight: 📖 RT‑SuperES builds on a legacy of single-molecule techniques. Read Sauer & Heilemann, Chem Rev for a deep dive into localisation microscopy evolution, a backbone to our live-cell approach. 🔗 pubs.acs.org/doi/10.1021/ac… #SuperResolution #RTSuperES European Innovation Council

📣 Did you know RT‑SuperES is funded by the European Innovation Council #PathfinderOpen programme? This support drives deep-tech projects that challenge today’s limits. We're building the future of real-time biological discovery. #RTSuperES #EIC #STEM

🧪 Partner spotlight: Prof. Ricardo Henriques Ricardo Henriques At @ITQBNOVA, his team pioneers adaptive microscopy and AI-enhanced imaging. In #RTSuperES, they develop real-time feedback pipelines that merge biology with precision optics. #AIinScience #Microscopy #PathfinderOpen

Meet device‑agnostic AI for super‑resolution imaging: reconstruct high-res images from a single diffraction-limited frame, no calibration needed. A major step aligns with RT‑SuperES goal of plug-and-play, high-speed live-cell imaging. #AIinMicroscopy #RTSuperES #MachineLearning

We’re looking for curious, innovative science leaders EMBL Heidelberg! Join a vibrant, interdisciplinary community where collaboration and innovation are nurtured at all levels. Take a look at these four open positions 👇

We’re often stuck looking at biology in snapshots, but life doesn’t work like that. At #RTSuperES, we’re building tools to watch epigenetic changes, protein relocalisation, and chromatin shifts in real time, as they happen, without the wait. #LiveCellImaging

Researchers at @isaaclilab developed qtPAINT, a molecular imaging method that measures forces inside living cells at nanometre precision, revealing mechanics in real time. A great complement to #RTSuperES and our AI-powered, live-cell imaging goals. #LiveCellImaging European Innovation Council

Saturday Delight: “Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications”. Clear guidance on resolution, artifacts, and reporting, timely for #RTSuperES. Read: pmc.ncbi.nlm.nih.gov/articles/PMC12… Lothar Schermelleh J Cell Science

Quick question for the community: which chromatin-associated protein would you HaloTag in mESCs to watch live (and why)? We’ll shortlist ideas for a future demo thread. #StemCells #Chromatin #RTSuperES European Innovation Council #PathfinderOpen Eran Meshorer

We’re building a library of HaloTag-tagged mESC lines so proteins are tracked endogenously in real time, with minimal perturbation, a backbone for #RTSuperES. #HaloTag #StemCells #Chromatin Eran Meshorer Hebrew University European Innovation Council #PathfinderOpen

Stunning STORM of intermediate filaments - excellent prep with the Smart Omnicell Kit on the SAFE platform by Abbelight. Robust sample workflows like this help power real-time, high-content super-resolution in #RTSuperES. #SMLM #STORM #Microscopy European Innovation Council #PathfinderOpen

Join Paola and team!🔥👇🔥

🔬 Meet 3I-SIM! Instead of stripe pattern, we use 3-beam to generate 2D hex pattern for simultaneous 2D SIM modulation, with >100,000 time-points, 13 h neuronal growth-cone movies, ER–lipid-droplet “track-switches” & millisecond tango. doi.org/10.1038/s41566… Nature Photonics

Fresh in Nature Photonics: 3I-SIM uses radially polarised interference to cut photobleaching and reach 242 Hz; with 3I-Net it holds ~100 nm over >100k frames. Kudos to corresponding author Peng Xi and team. #RTSuperES #SuperResolution #LiveCellImaging European Innovation Council #PathfinderOpen

Saturday Delight 📚 AI-assisted microscopy with pySTED: a STED simulator to train RL/DL and deploy on real scopes with no fine-tuning for autonomous imaging. Read: pmc.ncbi.nlm.nih.gov/articles/PMC11… FLC Lab #RTSuperES European Innovation Council #PathfinderOpen

👇👇👇

🔥 NEW SESSION ALERT! Hot Topics in Bioimage Analysis & AI is coming to #SPAOM 2025! 🤖🔬 Cutting-edge tools, deep learning, explainable AI & more — all in one session. 💥 Don’t miss this! 🚀 #AI #BioimageAnalysis #Microscopy #DeepLearning #ImagingScience #HotTopics