Reviewer: the sprouting of axon collaterals at nodes of Ranvier cannot be visualized. Me: Yes, they can. I see them everywhere, and you can as well if you have a better computer screen.

Reviewer: I did not see a single piece of data on the claims that their method can identify synapses. Me: You just didn't see a "Electron microscopy" kind of synapse.

As an engineer,I develop tools,suggest possibilities and ensure their quality. I don't mind the criticisms,they only take away the opportunity of the people who could be benefitted These authors got what they need with the LS18 tiling light sheet microscope and I got my royalty

😮~60% of us who had COVID still might have lingering viral spikes in our heads! Our new study reveals SARS-CoV-2 spike accumulation in the skull-meninges-brain axis & its implications in long COVID. By Zhouyi Rong HongchengMAI Saketh Kapoor, PhD🔬🧠🦠🧵👇 biorxiv.org/content/10.110…

1日で組み立て導入可能、実験台に乗るコンパクトさ、コストは300-500万（200万は消耗品）という、透明化技術ユーザーのためのライトシート顕微鏡 #descSPIM のプレプリントを、我々の研究室からの初めての論文としてbioRxivにサブミットしました！ #DBSB_Juntendo biorxiv.org/node/3126827.e…

Impressive, congratulations!

E8 mouse embryo. DAPI&Lectin, imaged using Nuohai LS18 tiling light sheet microscope.

The upgraded and redesigned LS18 tiling light sheet microscope to be distributed globally.

We studied the neuronal anatomy of planarian with single cell precision using tissue expansion and TLSM. We explored the possibility to illustrate the neural networks and regeneration using the latest tissue imaging techniques. Results are promising, but labeling is critical.

A few imaging results from the study. Supplemental video 3. 3D image of the visual projections in a wild-type planarian.

External and internal planarian muscles fibers. ps, we were trying to illustrate the locomotives neural pathway in planarians.

Supplemental video 9. 3D view of glial cell projection onto the body-wall muscles.

I think we understand tissue clearing and imaging much more than ~10 years ago. We can obtain results like below routinely. Making the tool more accessible and efficient, developing methods to use tissue structure data more efficiently appear to be major challenges in the next.

I propose a method to increase the imaging efficiency of tiling light sheet microscopy using scanning non-coaxial beam arrays synchronized with regional virtual confocal slits. It makes TLSM as fast as conventional LSM and keeps all rest advantages, but needs a new sCMOS camera.

The mechanism of the method. It should be extremely powerful if such a camera with multiple independent rolling shutters exists.

A non-coaxial beam array can be generated easily using a SLM and the pupil segmentation method. It allows a flexible control of each individual beam

The required sCMOS camera with multiple rolling shutters, i.e. multiple regional confocal slits.

There is a patented technology, invented by Bi-chang and me, to improve the imaging ability of lattice light sheet microscopy for large samples using tiling LLS . It can be conducted on any original design LLS microscope without hardware change. patents.google.com/patent/US20220…