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Happy to share our paper on long-term single-particle tracking in live neurons Nature Chemical Biology. We showed that the number of active dynein motors can switch stochastically during the long transport, and we resolved the molecular steps of dynein. nature.com/articles/s4158…

Recently out at Nat Chem Bio! I used my Ras biosensors (Zhang et al Nat Biotech 2024) to profile the activity and environment of Ras at the single-cell level, which unveiled the signaling mechanisms underlying RasG12C inhibitor resistance. nature.com/articles/s4158… Institute for Protein Design

A new paper captures the activation ensemble and associated dynamics of the adenosine A2A receptor-mediated coupling to cognate and non-cognate G proteins using 19F NMR combined with MD and Monte Carlo simulations. Free to read link at rdcu.be/dP8ur

A highly potent anti-liver fibrosis lead 12-deoxyphorbol 13-palmitate (DP) targets apolipoprotein L2 (APOL2) and impairs APOL2-SECA2-PERK-HES1 signaling. Read it here rdcu.be/dP8xT

A new paper reports the spatial regulation of metabolism in an E. coli community-level antibiotic response. Free to read link at rdcu.be/dQPSd

A new paper from Derek Sprayberry Aspacio et al. establishes a metabolic system of engineered biocatalysts using the noncanonical cofactor NMN+ for biomanufacturing in cell-free systems and in E. coli without interference from NAD(H) and NADP(H). Read it here rdcu.be/dQPUp

A new paper reported unidentified IscB proteins with editing activity in mammalian cells and developed one of them into a robust genome editing tool named IscB.m16* as well as base editors. Free to read link at rdcu.be/dR1EC

Spores harboring the gene circuit for secretory expression of Burkholderia cepacia lipase are processed with poly(caprolactone) to produce degradable living plastics. Read it here rdcu.be/dR1FO

Here, Khosla Lab and Cogan Lab use site-selective crosslinking to probe structural states that reveal how substrate carrier proteins interact with successive modules in representative assembly-line polyketide synthases. Free to read link at rdcu.be/dR1Nm

Artificial boron enzymes -- A News & Views by Yang Yang highlights recent work published @nature by Roelfes Group. Free to read link at rdcu.be/dR1PR and the original article rdcu.be/dR1PW

It's time for a small celebration! 🎉🎉😀🎉🎉 And if you like high-throughput assays and protein design, then join us for in this thread🧵 Massively parallel measurement of protein–protein interactions by sequencing using MP3-seq nature.com/articles/s4158…

Like a master craftsman, animals can mold basic materials into a variety of crystalline structures to perform functions ranging from pattern formation to camouflage and vision. The underlying mechanisms of this process have long remained a mystery. nature.com/articles/s4158…

Today in @natchembio we share a new strategy to design fully synthetic “artificial” bZIP family transcription factors that directly block DNA binding of HIF1a and XBP1 to inhibit hypoxia-dependent oncogenic signaling in cells and in vivo. 1/n  nature.com/articles/s4158…

Check out our latest article in Nature Chemical Biology where an intracellular micromap proximity labeling platform is established to investigate stress granule disassembly mechanisms. Congrats to Roderick, Steve and Sean! nature.com/articles/s4158…

Engineering artificial non-coding RNAs for targeted protein degradation. Read it here rdcu.be/dTsjJ

A ligand-based proximity labeling strategy enables mapping of the native cell membrane interactome for the GLP-1 receptor. Free to read link at rdcu.be/dTyui

Cryo-EM and biochemical analysis reveal the activation mechanism of protein arginine kinase McsB by its activator McsA for protein quality control under stress in Gram-positive bacteria. Free to read link at rdcu.be/dTyvl