The paper, reported by Drs Nishida and Shintani, identified antibiotics based on X-ray and cryo-EM structures of mitochondrial heme-copper oxidases. The structures were determined in SPring-8. KEK cryo-EM was used for the initial screening. Congrats!!! nature.com/articles/s4146…

We uploaded the following three textbooks for cryo-EM SPA initial training. Thanks! 1. Talos-Falcon4 in KEK (GUI: EPU) www2.kek.jp/imss/sbrc/beam… 2. Titan-Falcon4i in KEK (GUI: EPU) www2.kek.jp/imss/sbrc/beam… 3. CRYOARM300II-K3 in Tsukuba U (GUI: SerialEM) www2.kek.jp/imss/sbrc/beam…

The 19th paper from the #cryoEM facility in #KEK. Collaboration with Kawakami-san & Miyamoto-san in Keio U, Arai-san in Shinshu U. An artificial protein nanocage, mTIP60, can do the reversible assembly using metal ions. Its cryo-EM structure is beautiful! pubs.acs.org/doi/abs/10.102…

The 20th paper from the #cryoEM facility in #KEK. Collaboration with Drs Kosugi and Shibata. Went to the Antarctic, brought back an alga, and revealed its unusual LHC structure with C11 symmetry. A tiny amount, but enough to obtain its cryo-EM structure. nature.com/articles/s4146…

The 21st paper from the #cryoEM facility in #KEK. Collaboration with Prof Arimura. Using cryo-EM, they found multilamellar vesicle formation of squalene bearing 18-crown-6 ether (CESQ) depending on metal cations. Looks beautiful! pubs.acs.org/doi/10.1021/ac…

The 22nd paper from the #cryoEM facility in #KEK. Collaboration with Profs. Sawada, Sato, Fujita, and our 3DED/microED team. Crystal structures of de novo peptide fragments were determined by X-ray (tetrapeptide) and 3DED/microED (pentapeptide). Great! pubs.acs.org/doi/10.1021/ja…

The 23rd paper from the #cryoEM facility in #KEK. Collaboration with Profs. Ogawa. The two-step cargo recognition mechanism of heterotrimeric kinesin was revealed by SAXS, AFM, and cryo-EM. Fantastic! embopress.org/doi/abs/10.152…

The paper, reported by Prof. Fushinobu, gives structural insights into thermophilic chaperonin complexes. The structures were determined in SPring-8. KEK cryo-EM was used for the initial screening. Congrats!!!

The 25th paper from the #cryoEM facility in #KEK. Collaboration with Suzuki-Hamaguchi-san and Prof. Murata. 2.5A-resolution cryo-EM structure clearly shows P-gp with three elacriders and indicates a novel binding surface in P-gp. Interesting work! pubmed.ncbi.nlm.nih.gov/38579618/

The 26th paper from the #cryoEM facility in #KEK. Collaboration with Profs. Kawakami, Arai, Miyamoto. An artificial nanocage mTIP60 was split and re-designed to mTIP120, which obtained new N/C-termini serving for connecting fusion proteins. Beautiful! pubs.rsc.org/en/content/art…

The 27th paper from the #cryoEM facility in #KEK. Collaboration with Profs. Goto, Sato, and our 3DED/microED team. Structures of Indonesian traditional medicine, syzygioblanes A, were determined by the combination of microED, HRMS, and NMR. Great works! pubs.acs.org/doi/10.1021/ac…

SOUTH SUDAN vs USA | USAB SHOWCASE | FULL GAME HIGHLIGHTS | July 20, 2024 youtu.be/CEvcR5aQxI8?fe… YouTube

The 28th paper from the #cryoEM facility in #KEK. Collaboration with Profs. Awakawa, Mori, Abe. The β-NAD-complexed structure of PseP (pyridoxal-5′-phosphate-dependent enzyme (SbzP) homologue) was determined by cryo-EM. Really great!! nature.com/articles/s4192…

The 29th paper from the #cryoEM facility in #KEK. Collaboration with Prof. Murata. After the optimization of detergent, the preferred orientation was improved and the cryo-EM structures of hERG-inhibitor complex were obtained. Miyashita-kun, congrats! cell.com/structure/full…

The 30th paper from the #cryoEM facility in #KEK. Collaboration with Prof. Murata. The cryo-EM structure of V-ATPase and its inhibitor complex clearly visualizes the Na+ pathway! Fantastic!! nature.com/articles/s4159… pubmed.ncbi.nlm.nih.gov/39572733/

The 31st paper from the #cryoEM facility in #KEK. Collaboration with Prof. Kakuta. The cryo-EM structure of bacterial minimum RNaseP and pre-tRNA complex reveals structural basis of tRNA processing. Teramoto-san, Koyasu-san, congrats! nature.com/articles/s4146…

The 32nd paper from the #cryoEM facility in #KEK. Collaboration with Profs. Noji and Murata. Multi-stalk FoF1-ATP synthase was engineered to enhance H+/ATP ratio. Cryo-EM structures clearly showed the presence of multi-stalks! Outstanding work, Ueno-san! nature.com/articles/s4146…

The 33rd paper from the #cryoEM facility in #KEK. Collaboration with Prof. Miyanaga and Dr. Chisuga. Cryo-EM structure of modular polyketide synthases revealed by ancestral sequence reconstruction. Great!! nature.com/articles/s4146…