In this paper we report on 20 retrospective and 50 prospective intraoperative patient samples, providing a classification within 2 hours of sample receipt and a full molecular classification within 24 hours using the unique real time features of Oxford Nanopore sequencing.

OK -here we go again. Both flowcells were paused last night at around 1400 pores. Flushed, reloaded and restarted. They've each recovered to around 5,200 pores and are back sequencing. The new flow cell has approximately 60 Gb from 1 load cf 46 Gb on the standard with 2...

There's a buzz around Multi-pass, Single-Molecule Nanopore Reading of Long Protein Strands, a recent nature publication from Jeff Nivala, Keisuke Motone / 元根 啓佑, Daphne Kontogiorgos-Heintz and colleagues. Watch this publication highlight where they discuss their work! youtube.com/watch?v=aaE7kZ…

So 1/3 more data with 1/3 less DNA (and its still going). I suspect that the difference in N50 is a logical consequence of the reduction in blocking as molecules don't get stuck in the pore and produce noise? We also see fewer whale sharks (1mb+ reads that don't map!)

In the last few months at Oxford Nanopore, we've made major strides in improving the accuracy of native DNA sequencing in bacteria, a challenging task due to bacterial DNA modifications. Watch my colleague Katherine Lawrence dive into the details: youtube.com/watch?v=rRFREd…

Breakthroughs take a lot of dedication and hard work. It's a huge privilege to have some of these recognised. Congrats to the amazing Oxford Nanopore team who build our technology and to our fantastic customers who bring the to life! nature.com/articles/s4159…

How to call SNV in RNA seq from Oxford Nanopore or PacBio? Easy: Clair-3RNA biorxiv.org/content/10.110… ! ~95% and ~96% F1-score for ONT / PacBio + we detect RNA editing! Great collaboration with Ruibang Laurent Luo Sine zheng Miten Jain BCM HGSC Thanks to Genome in a Bottle!

SPECIAL ISSUE! This month Genome Research publishes a diverse collection of research and review articles in a special issue highlighting advances in long-read sequencing applications in biology and medicine. tinyurl.com/Genome-Res-34-….

Exciting news! The latest hifiasm release from Haoyu Cheng and Heng Li adds beta support for Oxford Nanopore simplex R10 reads. Initial results look very promising. 🚀 Check it out: github.com/chhylp123/hifi…"

Hifiasm 0.21.0 has been released. It now has a beta module for direct assembly of ONT R10 simplex reads. Initial tests with regular simplex reads show very promising results! github.com/chhylp123/hifi…

Our review on targeted long-read sequencing is part of the latest Special Issue from Genome Research ! If you have wanted to leverage long-read platforms for targeted applications but didn't know where to start, this review is for you! Full text: genome.cshlp.org/content/34/11/…

We’re delighted that @MitenJain (Assistant Professor, Northeastern University) will be joining the #sequencing roundtable at #FOG2025, helping you navigate the sequencing landscape in 2025! Register now: hubs.la/Q02Z1tN60

1/ 🧵 So excited to finally share our preprint! Long-read sequencing of hundreds of diverse brains provides insight into the impact of structural variation (SV) on gene expression and DNA methylation- A new genomic resource for brain research! 🧠✨biorxiv.org/content/10.110…

Huge performance update in today’s dorado v0.9.1 release on Ampere 8.6/8.7 & Ada Lovelace 8.9 NVIDIA GPUs with up to 2x increase in throughput with hac calling and 3x sup! 🚀 Plus a whole bunch of updates for dorado polish and the odd misc bug fix. github.com/nanoporetech/d…

And native barcoding for Oxford Nanopore direct RNA sequencing has arrived ahead of Oxford Nanopore Events. Very impressive first runs Genome Technology Lab with >25 million reads (pre-basecalling) per flow cell. Analyzed data forthcoming!

‘Why choose long reads versus short reads?’ Miten Jain (Associate Professor, Northeastern U.) poses this question in the latest edition of the Sequencing Buyer’s Guide. Hear what the experts had to say, download now: hubs.la/Q03m3D530

From the fantastic Matt Loose

A warm welcome to Miten Jain (Assistant Professor, Bioengineering & Physics, Northeastern U.) who will be joining a roundtable discussion on the future of genomic #sequencing at The Festival of Genomics & Biodata in Boston. More info: hubs.la/Q03kCn140 #FOGBoston

Announcing the Molecular Programming Decadal Flightplan! July 21–23 2025 | Seattle, WA We’re organizing a field-wide, community-driven conference to chart a 10-year strategic vision for molecular programming and we need you to help us lead it. Apply Now: mpflightplan.com/apply-now

New from the Rouhanifard Lab! What if we could sort single cells based on whether a specific gene was actively being transcribed—and then ask what drives that burst? Introducing: NuclampFISH