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We are thrilled to have Dr. Julie Theriot kickstart our online seminar series on the Physical Properties of the Cytoplasm next week. Visit cytophys.org for more info and Zoom links.

It is not trivial to make the right amount of histones in different nutrients, when #cellsize, growth rate, and S-phase duration change. Still, budding yeast manages by regulating transcription and translation: doi.org/10.1038/s44318… Francesco Padovani

Why are some cells bigger than others? Fascinating talk by Mike Lanz Stanford University Chan Zuckerberg Biohub Network investigating why cells are different sizes and how this relates to cell composition - using proteomic mass spec and validated in a range of cell types.

We’re hiring! We have Wellcome-funded postdoc positions to join my group. The project will build on recent work on transcriptional cell size scaling (Swaffer et al. Cell 2023) to ask how gene expression, RNA metabolism and cell signalling are all coordinated with cell size (1/3)

We will have Dr. Arthur Molines for an exciting talk next week! Please join us. cytophys.com

SpotMAX, our software tool for analysing multi-dimensional microscopy data, is finally out! 🎉 And conveniently, I just presented it at the #I2K conference 😀 A multi-year-long effort of many great collaborators and users. But what can SpotMAX do? A small thread

If your lab is using a Thermo LCMS consider our tool Rapid QC-MS for automated QC of internal and external standards. Our recent publication in Analytical Chemistry: pubs.acs.org/doi/10.1021/ac… Git: github.com/czbiohub-sf/Ra… Josh Elias Chan Zuckerberg Biohub Network #TeamMassSpec #OpenScience

Has scRNA-seq transformed our understanding of how complex tissues are structured ?

Excited to share our preprint reporting a completely new approach to study kinase signaling (ProKAS): "Proteomic Sensors for Quantitative, Multiplexed and Spatial Monitoring of Kinase Signaling" We applied it to DDR kinases. Please share. biorxiv.org/content/10.110…

Please spread the word

What determines protein levels ? For many proteins, abundance variation is dominated by clearance (degradation & dilution) variation. Globally, clearance variation across tissues (e.g., brain & pancreas) explains ~ 40 % of protein abundance variation. biorxiv.org/content/10.110…

Here is the link to the final version of our manuscript on K63-polyubiquitin and ATG9A in membrane trafficking.(molbiolcell.org/doi/10.1091/mb……)

Our paper on the impact of protein leakage due to loss of membrane integrity for single cell proteomic sample prep is out in Nature Communications! We analyze thousands of primary single cells from murine tracheal tissue and record membrane permeability🧵 nature.com/articles/s4146…

DR5 is a favorite auxin reporter. In Nature Communications we show DR5 expression is stochastic, exhibiting both intrinsic and extrinsic noise. We put two different color DR5 reporters in the same plant and see stochasticity. Shuyao Kong @mingyuan2328 @byron_rusknak rdcu.be/emNVQ

Establishment of cell size–dependent growth rate via differential scaling of metabolite uptake and release | PNAS pnas.org/doi/10.1073/pn…

Just out! Aneuploidy alters cellular proteome and affects many functions. One protein, p62/ SQSTM1, becomes highly abundant and creates cytosolic bodies in cells with extra chromosomes. nature.com/articles/s4146…

Delighted to share our latest work showing how cells that evade #senescence after CDK4/6 inhibition are primed to become aneuploid because of their increased size. Great work by super PhD student Aanchal Pareri biorxiv.org/content/10.110…

The big one is finally out!! In this paper, we set out to provide insight into the fundamental question; How do the individual cells from complex tissues regulate their proteomes? Brief summary of our findings 👇 biorxiv.org/content/10.110…