Here's more on Pol IV-RDR2 coupling, highlighted by the spectacular new Science paper from the Zhang lab and new PNAS paper by Fukudome et al. in my lab. Here is our version of the model for how Pol IV arrest and backtracking enables RDR2 synthesis of the 2nd RNA strand.

Benchmarking Glacios, which was recently installed. The numbers look good to me. I love Falcon 4 detector

New Pikaard lab paper, showing how alternative dicing patterns are programmed for maximal siRNA-directed DNA methylation, just published in eLife. Kudos to former PhD students Loffer and Singh and postdocs Fukudome, Mishra and Wang doi.org/10.7554/eLife.…

We just have completed cryo-EM training assisted by Matthjin Vos 😊

We’re excited to announce that David Agard, Bridget Carragher + Clint Potter have joined our new Chan Zuckerberg Initiative Imaging Institute to bring together scientists and engineers to develop new cryoelectron microscopy imaging hardware and software 🧵czi.co/ImagingInstitu…

🔥hot off the press from the Liz Kellogg ❄️🔬 lab 🔥 We report the first cryo-EM structure of a CAST catalytic component, commonly called the transposase. In the V-K CAST system this component is named TnsB, published this week in PNAS! (1/9) pnas.org/doi/full/10.10…

Finally able to kick off the Cold Spring Harbor Laboratory cryoEM course originally scheduled for March 2020! It’s great to be back

We are delighted to share our work, now available in nature: nature.com/articles/s4158… Our scoring system can be accessed here: kinase-library.phosphosite.org Coming soon --> the tyrosine kinome and an improved system with many new functionalities & features! #TheKinaseLibrary #KL

I am delighted that my lab was able to contribute to a large kinase substrate study with other excellent groups just published in nature 😊: nature.com/articles/s4158…

Looking for a postdoc to work on small molecule degraders of oncoproteins (Bellail et al. Science Translational Medicine 2021, 13:e12947, DOI:10.1126/scitranslmed.abh1486), and aging related proteins utilizing cryo-EM If interested, please contact me at [email protected]

Our recent work has been deposited into bioRxiv. A great collaboration with the groups of Zhu, Richards, and Chaptal 😊😊: biorxiv.org/content/10.110…

can't wait to join in on the 🔥🔥🔥 #cryoEM program led by Yifan Cheng and superstars Michael Cianfrocco Chi-Min Ho @fitzpatricklab Dave Taylor @YuanHe_NWU @Mel_Ohi_Lab Alexis Rohou Brent Nannenga Anchi and more at #ACA2023.. 'American Cryo-EM Association'!! 😂

Just finished CryoEM and ET workshop at ACA meeting. Thanks for outstanding speakers 😊👍

Nice to see Mike in person

Matthijn Vos is evaluating student grids at the ⁦CSHL Courses⁩ cryo-em course. He is a tough judge - a lot of 0/10 grids! ⁦Lander Lab⁩ ⁦Jennifer Cash⁩

Thrilled to share that our paper has finally been published after navigating feedback from six reviewers over the past year and a half. Huge thanks to the incredible collaboration with the teams of Richards, Zhu, Vos, and Chaptal. rdcu.be/d2iMK

Working on ASNS, authors from Takagi_Lab Wen Zhu Lab and colaborators combine cryo-EM 3D variability analysis with MD simulations to study conformation of amino acid side chains. nature.com/articles/s4146…

1/2: Publication Announcement! In a new manuscript published in Nature Communications, Dr. Yuichiro Takagi Takagi_Lab and collaborators used Cryo-EM to reveal the catalytic mechanism of Human Asparagine Synthetase (ASNS): nature.com/articles/s4146…

My cat passed away yesterday at nearly 18 years old. Many of you knew her as the lab’s quiet mascot—her photo appears on our X account and elsewhere. She had been with me through most of my time at IU, offering steady companionship. She will be greatly missed.

I am excited to share our latest work, now available on bioRxiv: biorxiv.org/content/10.110… This achievement was made possible through great collaboration with the Staschke (IUSM) and Zhu (FSU) labs.