The latest #cellfree natural products work from my lab! nature.com/articles/s4159…

Take a look at our research on Human Milk Oligosaccharide being covered in the .The Guardian ! theguardian.com/science/articl…

Congrats to our very own @allie_npearson for winning Best Poster Award at the @ ECI Biochemical and Molecular Engineering XXIII in Dublin, Ireland 🇮🇪 on her work uncovering the genetic basis of dicarboxylic acid metabolism. We are so proud! 💚🤍🧡

The Shih Lab is interested in .UC Berkeley undergraduates who are interested in working with fungi in our lab located at Joint BioEnergy Institute (JBEI)! If you are interested please DM us!

Binary vector copy number engineering improves Agrobacterium-mediated transformation go.nature.com/3CdewQS

I am pleased to announce new research from the Shih Lab (ShihLab) published today in Nature Biotechnology on improving Agrobacterium-mediated transformation via binary vector copy number engineering (doi.org/10.1038/s41587…). 🧵A summary thread below🌿

In Science Advances, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains science.org/doi/10.1126/sc… (1/n)

New OA Article: "Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism" rdcu.be/elJ5z News & Views: "Cooperation and antagonism in Agrobacterium-mediated transformation" rdcu.be/elJ5F

nature.com/articles/s4147…

We're excited to share another work from our lab this week. We present ENTRAP-seq, the first massively parallel reporter assay to characterize protein-coding libraries in plants. Congrats to Simon, Lucas, and all other co-authors. biorxiv.org/content/10.110…

Today the Shih Lab (ShihLab) presents ENTRAP-seq, a high-throughput assay for functional screening directly in plant cells.

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression biorxiv.org/content/10.110… #biorxiv_plants

Check out our new preprint! We examined sources of variability of transgene expression in N. benthamiana agroinfiltration and ways to minimize it. Graduate student Sophia Tang observed FP expression in >1,800 plants over the course of nearly 3 years! biorxiv.org/content/10.110…

A truly enabling new approach by Tara Lowensohn, Will Cody and Sattely_lab sattelylab.bsky.social to probe plant genetics at scale. Single-gene-per-cell delivery coupled to an effective transcriptional selection system. Libraries in plants. biorxiv.org/content/10.110…