Humbled and incredibly honored to be selected to the 2023 class of Jane Coffin Childs Fellows @JCChildsFund, sponsored by HHMI HHMI . Huge thanks to my Postdoc mentor Dr. Jennifer Doudna @doudna_lab at Institute for Geographic Information Science, my PhD mentor Dr. Hashim Al-Hashimi and all my dear friends. 😀

Was great to show our work in @doudna_lab (+ Amy Lu Seyone Chithrananda who couldn't make it) on protein homology search today ICML Conference ICML-Compbio-Workshop! Full poster in thread below 👇

Check out our paper and the accompanying perspective by Kuhlman published today in Science Magazine about the surprising role of short tandem repeats in regulating eukaryotic transcription! science.org/doi/10.1126/sc… science.org/doi/10.1126/sc… A thread (1/11):

Spurred on by ribosome profiling and other cool methods, translation elongation has grown into a whole field with new surprises all the time (and new relevance for vaccine mRNAs!). Here’s our latest on why synonymous codons aren’t all the same. (Thread) biorxiv.org/content/10.110…

In vivo human T cell engineering with enveloped delivery vehicles go.nature.com/47vW1Rp

We’ve got a guest submission!! Guess this spill kit is only good for organisms with left handed DNA…

How genome editing can be more efficacious and accessible in the future. Delivery of editors without virus vectors. pnas.org/doi/10.1073/pn… PNASNews @doudna_lab Connor Tsuchida UC Berkeley

The future of genome editing is in vivo! Check out our latest PNASNews perspective from @doudna_lab. Had a lot of fun discussions with Kevin Wasko and Jennifer R Hamilton about the past, present, and future of in vivo delivery. pnas.org/doi/10.1073/pn…

📈 Trending Article in PNAS One of the most-viewed PNAS articles in the last week is “Targeted nonviral delivery of genome editors in vivo.” Explore the findings here: ow.ly/vCwS50Rh306 For more trending articles, visit the PNAS homepage at ow.ly/pC3T50Rh307.

Thrilled to share our first preprint! We present comprehensive protocols for delivering CRISPRoff as plasmids or mRNA into cancer cells and primary human T cells, enabling durable and heritable gene silencing! biorxiv.org/content/10.110…

🚨 New preprint alert! I am excited to present a collaboration between the Doudna Lab and Zev Bryant lab, with co-first authors Noor Al-Sayyad and Kevin Wasko and me. Curious about what drives high-efficiency genome editing by Cas9? Let’s dive in!👇🧵biorxiv.org/content/10.110…

How does Hachiman work, and what can it teach us about immunity? Our structural and functional characterization of the Hachiman defense system is online: sciencedirect.com/science/articl… Thanks to co-lead Ben Adler, Emily Armbruster & coauthors at @doudna_lab. Institute for Geographic Information Science College of Chemistry HHMI

Thrilled to share our latest work on lipid nanoparticle (LNP) delivery of a stable CRISPR-Cas9 ribonucleoprotein (RNP) for in vivo genome editing, now published Nature Biotechnology! 🧬 nature.com/articles/s4158… In this paper, we first engineered robust, efficient, and stable

Our paper is finally out in Molecular Cell! 🚀 We dissect how Cas9 variants like SpRY lose efficiency by getting kinetically trapped during target search. Mechanistic insights to guide the next-gen genome editors. Huge thanks to all co-authors! #CRISPR #gene_editing🔬🧬