1/ Excited to share our protocol for the high-throughput PAM determination assay (HT-PAMDA), a method for PAM characterization of hundreds of Cas enzymes in parallel. Ben Kleinstiver Jonathan Hsu J. Keith Joung nature.com/articles/s4159…

In the words of Eminem, here I go with my shot. H/t Randall Munroe

Congrats to first-author Esther Tak Y. Esther Tak and co-authors (Joy E. Horng Nicholas Perry Hayley Schultz Sowmya Iyer Qiuming Yao Luli Zou Martin Aryee Luca Pinello) on the publication of our newest paper in Nature Methods (free link: rdcu.be/csoDt). Will tweet summary soon

Thrilled to share (a few days late) our work describing how to more consistently activate human genes from enhancer and other distal sequences using CRISPR activators (published in Nature Methods nature.com/articles/s4159… free link: rdcu.be/csoDt ). In it, we tackle..1/9

"Wait, you have a wet lab?" Come for the overview of patient-derived cellular models in Biological Psychiatry Society of Biological Psychiatry by Steve Sheridan, stay for the dancing microglia by Joy E. Horng biologicalpsychiatryjournal.com/article/S0006-…

Excited to share that our collaboration with Miguel Rivera's lab MGH Pathology using engineered zinc finger transcription factors to induce genome-wide perturbation of microsatellite repeats is out today in Cell Genomics. cell.com/cell-genomics/… Summary tweet thread coming soon.

Extremely excited to kickstart my lab TU München with Emmy Noether support from DFG public | @[email protected]. I’m very grateful to J. Keith Joung, my friends in Boston, and to A. Moretti, K.L. Laugwitz & C. Kupatt in Munich. More details & website coming soon. #CRISPR 🫀

Our new preprint describes “CROPseq-multi”: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens doi.org/10.1101/2024.0… Reagents on Addgene: addgene.org/browse/article…

We're hiring Research Associates/Techs Arena BioWorks to work on gene editing tech dev projects. As it was for many who worked in my former MGH Pathology lab, it's an opportunity to gain outstanding scientific training & mentoring for graduate school. linkedin.com/jobs/view/3860…