Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors mdpi.com/2526086

We present projective oblique plane structured illumination microscopy (POPSIM): nature.com/articles/s4430… POPSIM allows imaging of an entire cell under structured illumination using nine 2D projections, and as such presents a rapid alternative to conventional 3D stacking in SIM.

#AIRR microscopy has arrived! Use any immersion objective (including air!) to look into any sample refractive index! Built here with air, water and oil objectives, and for a sample RI in the range 1.33-1.51 (zoom lens). Could this be the fastest/most versatile light-sheet ever?

The uropod of the amoeba Chaos is labeled by microinjecting Lifeact-GFP2 protein. The #AIRR Single Objective Light Sheet by Alfred Millett-Sikking and the team at Calico is a game changer for depth and speed! We imaged approx. 250x320x100um^3 volumes in <1s using a 40x 0.95 NA objective.

We are hiring! If you are excited about building microscopes, voltage imaging, or techniques that can improve biological measurements I'd love to hear from you.  Postdoc ad linked below, and we will be hiring at all levels soon. recruiting2.ultipro.com/SAL1013SIBS/Jo…

Happy #FluorescenceFriday! Out now is the latest work from my PhD with @millerchembio showcasing two chemical-genetic targeting systems for VoltageFluor dyes, enabling cell-type specific monitoring of membrane potential – read out optically with FLIM. tinyurl.com/5n8zhjdh

RESOLFT was never faster! Happy to share the OPM with @snouty and estefani raquel to craft several thin sheets of light at the same time and read them out really fast! Congrats to Andreas Bodén Dirk Ollech and the snouty family Andrew York and Alfred Millett-Sikking

Attention organelle enthusiasts!! I am beyond thrilled to introduce Nellie: a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D/3D live-cell microscopy. paper: arxiv.org/abs/2403.13214 github: github.com/aelefebv/nellie 🧵1/N

Happy #FluorescenceFriday everyone! Our "Renaissance Dye" CRhOMe (and my co-first author paper from my PhD) is now published and online at PNAS: pnas.org/doi/10.1073/pn… Really proud of this work and everyone from the @MillerChemBio lab who contributed to this project.