CRISPR #geneediting holds huge therapeutic promise. How good are the various CRISPR tools, and how safe? In this review, our own Jianli Tao, Daniel Bauer & Roberto Chiarle summarize the capabilities and limitations of each: ms.spr.ly/6018eAGpn

#CRISPR tools compared: In this review in Nature Communications, Jianli Tao, Daniel Bauer & Roberto Chiarle look at CRISPR-Cas9/Cas12/Cas13 nucleases, DNA base editors, prime editors, and RNA base editors and offer considerations for therapeutic gene editing. ms.spr.ly/6010eAGZi

.Jianli Tao Daniel Bauer Chiarle lab review the development of CRISPR-Cas nucleases, base editors, and prime editors, as well as their editing precision, off-target effects, and clinical considerations

go.nature.com/3XKXC1u

REVIEW 'Assessing and advancing the safety of #CRISPR -Cas tools: from DNA to RNA editing'
Tao J Jianli Tao, Bauer DE, Chiarle R.
Nat Commun. 2023-01-13. (10 pages, 247 references)
doi.org/10.1038/s41467…

Thank you David and Peter, to include our work in this amazing review. The message is 'DSBs generated by nucleases can cause large deletions, chromosomal translocations, chromothripsis, retrotransposon insertion and activation of p53, which can enrich oncogenic cells.'

Our team on the Bridgeport, CT to Port Jefferson, NY ferry. Road-tripping from ⁦Dana-Farber⁩ to ⁦Cold Spring Harbor Laboratory⁩ #cshlTE22 . ⁩

A caution on CRISPR in Nature Communications, and the suggestion that base editing and prime editing may be safer from Jianli Tao, Roberto Chiarle, et al. ms.spr.ly/6017b40J9 #mobileelements #jumpinggenes

A potential danger of #CRISPR gene editing—and why base editing may be safer @bostonchildrens Nature Communications doi.org/gqf9x7 phys.org/news/2022-07-p…

New work by @Jianli_tao et al. compare insertions of LINE-1 retrotransposons in human cells during editing by CRISPR-Cas9 nuclease, base editors, and prime editors. They observe >2,500 de novo L1 insertions from Cas9 nuclease, but few from CBE, ABE, or PE.
nature.com/articles/s4146…

Our work on LINE-1 insertions at CRISPR editing sites is already out! Congratulations to Jianli Tao (Jianli Tao) and Qi Wang (@flying_gene) Chiarle lab. Many thanks to our collaborators from Kathleen Helen Burns to help to make it possible.

Our work finally published Nature Communications with our amazing collaborators Kathleen Helen Burns @_carloshhh Thank you Ross Cloney for all your great efforts.

And Jianli Tao Qi & co in Chiarle lab at Boston Children's Harvard Medical School explore the frequency & mechanism of LINE-1 insertions at #CRISPR editing sites

go.nature.com/3OWadKC