UConn Advanced Light Microscopy Facility
@uconnalmf
The ALMF is a part of the Center for Open Research Resources and Equipment at UConn. We provide microscopy expertise and access to cutting edge instruments.
ID: 1569413872490270723
http://confocal.uconn.edu 12-09-2022 19:53:28
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New instrument alert! Our newest addition, the Nikon Instruments point-scanning AXR #confocal, also equipped for TIRF, is up and running! Full information about the instrument and use/trainings can be found here: confocal.uconn.edu/nikonaxr/
Happy #FluorescenceFriday! The ALMF Core has been busy the past few weeks, so we have some repeat images collected on our Leica Microsystems SP8 with different LUT displays. We're also interested in some robust specimen slides for practice, so if anyone has recs please comment 👇
Having fun with our new Nikon Instruments AXR confocal and using the NIS Elements movie maker function. This is a 3D reconstruction of the crypts in a mouse intestine section showing goblet cells (blue), nuclei (green), and actin (red).#confocal
The value of machine learning using ZEISS arivis 🔬 software for segmentation becomes clear in a sample with high dynamic range, such as this mitotic spindle (bottom). Most of the dimmer astral microtubules are lost with traditional segmentation algorithms (top).
Happy #FluorescenceFriday! We've been playing around with large image + z-stack using JOBS on the Nikon Instruments AXR, which is super useful! Image 1 is a 2D overview, and 2 & 3 are max projections from select regions of this kidney section at 40X.
Happy #MicroscopyMonday! Here we have a fern rhizome imaged with a color camera on a Leica Microsystems Thunder without (Left) and with (Right) a DIC prism in the lightpath, creating interference that results in other colors.
This #FluorescenceFriday we bring you some fun #sciart of a "microscopic sunflower," found from a hibiscus sample! Captured using green and far red fluorescence (yellow and orange here, respectively) and DIC on our Leica Microsystems Thunder. Any #plantsci people able to ID this?
Double dipping on this same stellate trichome (left) for #microscopymonday! This image is using the Leica Microsystems Thunder K5C color camera and applying extended depth of focus. We also imaged part of the stem itself (right) and found other interesting structures!