Our platform for rapid manipulation of endogenous proteins at scale #SPOTLITES can be employed to discover novel effectors for #targetedproteindegradation #TPD and other proximity-based approaches! biorxiv.org/content/10.110…

I'm really excited to present work from Ophir Shalem’s group with my outstanding co-first author Yevgeniy Serebrenik Yevgeniy Serebrenik to tag and manipulate proteins at scale, which we use to study the #proteome and #proteostasis biorxiv.org/content/10.110…

#SPOTLITES (‘Scalable POoled Targeting with a LIgandable Tag at Endogenous Sites’) would not exist without our fantastic co-authors Tomer Lapidot, GB, and Ophir Shalem. Thanks also to CHOP's CAG sequencing core, the optical sequencing community, and our wonderful labmates!

Happy to share two preprints where we demonstrate Scalable POoled Targeting with a LIgandable Tag at Endogenous Sites (#SPOTLITES) for protein imaging, perturbation and recruitment. Led by v v talented Yevgeniy Serebrenik and Steph Sansbury biorxiv.org/content/10.110… biorxiv.org/content/10.110…

Fantastic collaboration with GB lab. Special mention for Tomer Lapidot for excellent and extensive computational work and high throughput image analysis and Deepak Mani that led many of the experiments in the recruitment paper