🎯#dfPPI employs protein complexes that form under stress conditions as biological baits, capturing disease-specific changes in protein interactions within native cellular environment. This improves relevance & accuracy of the findings in biological contexts. #Chemoproteomics (3)

🔍Specialized #ChemicalProbes are central to dfPPI, capturing dysfunctional interactions—trapping thousands of protein interactors, enabling precise identification of disease-driven interactome changes. #MassSpec #Proteomics (4)

🌐The open-access dfPPI Platform lets researchers worldwide visualize and analyze dfPPI data interactively, empowering researchers with access to cutting-edge proteomics analysis. #OpenScience #Proteomics (10)

🚀 Integrating dfPPI with other omics could reveal comprehensive disease mechanisms, paving the way for groundbreaking discoveries and precision therapeutics across numerous diseases. #MultiOmics #FutureResearch #Omics #dfPPI #SystemsBiology #Bioinformatics (11/11)

🧵Follow this thread by Tanaya Roychowdhury to learn about the intriguing case of #HSP90 - adapted from our recent publication in Nature Communications (1/8) #cancer #phosphorylation #epichaperome #conformation #cellstress nature.com/articles/s4146…

Heat Shock Protein 90 (#HSP90) is a cellular chaperone protein ubiquitously present in human body and is responsible for folding other important proteins in the cell. However, it can exist either as Dr. Jekyll or Mr. Hyde depending on how stressed the cells are. (3) #cellstress

#Cancer is a multifactorial disease that manifests as a result of chronic cellular stress, a trigger that turns HSP90 into Mr. Hyde. Mr. Hyde can now associate with other chaperone protein members and form a gang (high molecular weight assembly) called “#epichaperome”. (5)

This switch of HSP90 to "bad" and the formation of the epichaperome makes it so the #epichaperome now aids #cancer. (6)

In cancer, Casein Kinase 2 (CK2) interacts with #HSP90 and puts phosphate groups on Serine 255 and Serine 226 positions of the charged linker region of HSP90 thereby changing its structure or conformation. This dangerous alter ego of HSP90 helps the cancer cells to survive. (7)

To know more about this strange and intriguing case check out our work linked below (8/8) #phosphorylation #epichaperome #cancer #conformation #cellstress nature.com/articles/s4146…

@ChiosisLabMSKCC sushi dinner a few weeks ago! It is always so nice to gather as a lab for dinner - this time we were joined by a collaborator and friend, Hediye, and a couple of our summer interns! 🍣😋

Are you attending #ACSFall2025? Keep an eye out for Chander S Digwal, he will be presenting Targeting Aberrantly Glycosylated GRP94: Conformational Dynamics and Implications for Drug Design early next week! #GRP94 #epichaperomics #glycosylation #proteinproteininteraction #PPI

✨Today's the day! If you're at #ACSFall2025, don't miss this poster session 🧪

Online now - PTMs as molecular encoders: reprogramming chaperones into epichaperomes for network control in disease dlvr.it/TMkyft

Fantastic review by Gabriela Chiosis and team connecting the epichaorome with the chaperone code

Chiosis Lab Trends in Biochemical Sciences Dive deeper into the concept behind our Trends in Biochemical Sciences TIBS cover article! Read more on Springer Nature Communities communities.springernature.com/posts/when-mod… #Epichaperome #PTMs #NetworkBiology #SystemsBiology #TrendsBiochem #NatureCommunities

How can post-translational modifications rewrite cellular networks? Read communities.springernature.com/posts/how-post… Springer Nature Trends in Biochemical Sciences #Epichaperome #PTMs #NetworkBiology #SystemsBiology #MolecularChaperones #CellBiology #Neurodegeneration #CancerResearch

Proteins rarely work alone. Their functions emerge from networks of interactions—dynamic connections that continuously reorganize to maintain cellular balance. What happens when this balance is lost? This blog dives into our Trends in Biochemical Sciences review article: communities.springernature.com/posts/how-post…

In our recent Trends in Biochemical Sciences review, we propose that #PTMs do far more than modulate single-protein behavior. Further explore how #posttranslationalmodifications can rewrite cellular networks➡️Springer Nature Communities: go.nature.com/4o8jGQD #epichaperome #networkbiology

📸OUT NOW in JoVE: Mapping Dysfunctional Protein-Protein Interactions in Disease. Our method article is available now with video component coming soon! app.jove.com/t/69197 #PPI #networkbiology #cancerbiology #dfPPI #MassSpectrometry #Proteomics