High-resolution data for beam-sensitive nanocrystals doi.org/10.1107/S20522… U of T Physics University of Toronto MPI für Struktur und Dynamik der Materie Stockholm University Chemistry at UofT Vetenskapsrådet NSERC / CRSNG KAW #electrondiffraction #electronmicroscopy #nanocrystallography #zeolite IUCr #openaccess

A new approach for serial electron diffraction data collection IUCrJ doi.org/10.1107/S20522… SEMTEArizona State University Biodesign Institute at Arizona State University @ASU #serial #electrondiffraction #scanning #transmission #electronmicroscopy #nanocrystallography #beamsensitive #zeolite IUCr #openaccess

The future of biology may be cryo-ptychography, the technique with the highest resolution currently in materials science at the picometre level. Potentially with a log fold less particles than currently, just a few 10s of k particles.

Excited to share our latest work in Cell on how bluetongue virus packages its RNA & assembles its capsid inside host cells! Using #cryoET & #cryoEM, we've visualized this process with 11 structures. Great collab w Tamir Gonen The Zhou Lab 🦠🔬 cell.com/cell/abstract/…

I am excited to share that I will be opening my own lab at Hauptman-Woodward @HWMRI in Buffalo NY this summer! I am grateful for the incredible journey and support from Tamir Gonen and UCLA Biological Chemistry. The new lab will be harnessing #CryoEM and #MicroED to explore antibiotic resistance.

In three weeks, I am going to start my own research group at ANU Research School of Chemistry ourANU to continue the development of electron crystallography methods, including #3DED/#MicroED and #SerialED. I would like to thank everyone who has helped and influenced me along the way.

Finally published! A really interesting project in which got to know many great collaborators. Together we studied the hemozoin structure in incredible detail. It took a long time to get here though - my first notes for this project are pre-Covid... doi.org/10.1021/acscen…

Big news soon for #cryoem macromolecular #MicroED...watch this space 😳

Now online: Article by Hai-Sen Xu, Yi Luo, Tom Willhammar, Kian Ping Loh, Cheng-Yong Su & co-workers Hierarchical assembly of tubular frameworks driven by covalent and coordinate bonding nature.com/articles/s4416… ($)

#cryoEM with energy filter & electron counting significantly increased resolution even for a full size protein like proteinase K. Sub-atomic resolution #MicroED rivals best reported Xray of Proteinase K but by using crystals a billionth the size. A section of the map is shown

Excited to share our latest preprint Tamir Gonen Mike Martynowycz, where we demonstrate how an energy filter can significantly enhance macromolecular MicroED data quality - showing diffraction spots extending to ~1 Å resolution from proteinase K xtals: doi.org/10.1101/2024.0…

👀❄️🔬Check it out from Jae Yang, Josh Mitchell, Craig Bingman, Deane Mosher, & me!! Tour de force of #cryoEM, #microED, #cryoET, & #cryoFIB to solve the in situ crystalline structure of the #Eosinophil major basic protein-1! biorxiv.org/cgi/content/sh… Congrats! ⁦UW–Madison Biochem⁩

Excited to share our new work! We shed light on the sociology of virus-induced condensates supporting reovirus assembly and replication. Visualized using #CryoET #CryoEM and #FIBmilling nature.com/articles/s4146… Great collab w/ xian xia The Zhou Lab Xiaoyu Liu Tamir Gonen

Glad this is finally out! A group effort of multiple generations of Gonen lab alumni! If you want a step by step guide on all the microED variants possible. This is your bus stop! 🎉🎉🎉 Happy to answer any questions also by email or DM :--) nature.com/articles/s4159…

Now online & open access: Article by Yi Luo, Hao Xu, Yue Han, Xiaodong Zou, Peng Wu & co-workers Xiaodong Zou Lab Atomic-scale insights into topotactic transformations in an extra-large-pore zeolite using time-resolved 3D electron diffraction nature.com/articles/s4416…

Now online: News & Views on the Article by Yi Luo, Hao Xu, Yue Han, Xiaodong Zou, Peng Wu & co-workers Xiaodong Zou Lab nature.com/articles/s4416… Deciphering the dynamics of topotactic transformation by Jeffrey Rimer UH_RimerGroup nature.com/articles/s4416… ($)

Xiaodong Zou & colleagues Stockholm University, East China Normal University & Sinopec Shanghai Research Institute of Petrochemical Technology have published in Nature Synthesis on how to unlock secrets of zeolites, materials crucial to many industrial processes su.se/english/news/u…

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in Cell cell.com/cell/fulltext/… . What’s new?

Our latest work shows how energy filtering, PFIB, and direct electron detection unlock sub-atomic resolution for #MicroED! 🎯 Great work from Max Clabbers and JohanHattne back when I was with Tamir Gonen at UCLA Biological Chemistry. Excited to bring this capability to UBuffalo!

Our latest manuscript is out! We combined direct electron detection with energy filtering to boost the SNR of our MicroED data This work was done at HHMI and UCLA with Mike Martynowycz who now runs his own lab at UB-HWI, Johan Hattne, and Tamir Gonen nature.com/articles/s4146…