Interested in exciting new #membrane trafficking research and discussions? There's a Slack for that! Organized by Fran Bottanelli (FU Berlin), Ishier Raote (CRG, Barcelona) and Felix Campelo (ICFO, Barcelona). Join at: ow.ly/hzR850zLAVk

Check out our short review about #ARF #GTPases. ARFs are master regulators of trafficking and we still have so much to discover about their cellular functions! We also share some of our views and opinions. Great work by two PhD students Luis Wong-Dilworth and Petia Adarska!

Manuscript uploaded to BioRxiv 😎 Awesome gene editing and #STED tour de force by Luis Wong-Dilworth and many others!

Very excited to have this out! Here we use #CRISPR and #STED #superres microscopy to map the nanoscale localization of ARF GTPases! With many surprises! Twittorial this evening 😊

And as promised here is a spontaneous Twittorial about our latest preprint. I have always been fascinated by ARF GTPases, they are really masters of the cells regulating #membrane homeostasis, #metabolism, #cytoskeleton. Humans have 5 ARFs and WHY DO WE NEED SO MANY?

Tune in this Thursday when I will talk about our work on #geneediting and live-cell #STED to understand the function of #ARF #GTPases in living cells! Pre-print biorxiv.org/content/10.110…

Paper accepted celebrations! 🤩 good job Luis Wong-Dilworth and all co authors!

A tour the force of #geneediting and #STED. We have followed up on some of these findings and have 2 papers that will drop soon! looking at endogenous proteins at high resolution in their own unperturbed cellular environment is helping to reveal important trafficking mechanisms!

STED imaging of endogenously tagged ARF #GTPases reveals their distinct nanoscale localizations, say Luis Wong-Dilworth @freethepipette, Francesca Bottanelli @fran_bottanelli and colleagues #microscopy

Luis Wong-Dilworth @freethepipette, Francesca Bottanelli @fran_bottanelli et al. use STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases, suggesting distinct functions for ARF paralogs in intracellular #trafficking. bit.ly/3HhBHcp

Cracking the ARF GTPases code. Thank you for the great opportunity Francesca Bottanelli and to all members from the lab that made this possible. Excited to learn the insights that follow from this work!

3 first authors! 2 papers! Looking forward to getting feedback from the community! Alexander Stockhammer Petia Adarska Luis Wong-Dilworth

Terrific preprint from Francesca Bottanelli! ER-to-Golgi transport via an ARF4 tubulo-vesicular network connecting ER and Golgi-associated pre-cisternae Nanoscale imaging reveals the mechanisms of ER-to-Golgi transport via a dynamic tubular-vesicular network biorxiv.org/content/10.110…

Your TubER driver has arrived. Check out the latest insights into ER-to-Golgi traffic from the Bottanelli Lab! Francesca Bottanelli biorxiv.org/content/10.110…

Tubules are the new headliners today!

More tubular networks also in plants!