Checkout the recent reposts at imagingartifact.bsky.social

Having some fun with my eBIC Beamline #cryoEM ❄️🔬session with SPA EPU's acquisition templates: 9-13 shots/hole 🔥 on Quantifoil (Au) R2/1. In the end we will take the recommendation of our local contact and collect sensibly.

The new long duration dewar to run cryoFib milling overnight is massive !!

Boo!

finding blackholes in my cryoEM dataset

This illustrates a very coherent point

After 20 Years of services in biological research in Rennes and almost 7 Years of imaging many samples at the EM facility, it is time for you to go. So long old friend. Thank you for those wonderful Years !

SPACEtomo v1.2 is online! github.com/eisfabian/SPAC… - Improved GUIs for lamella detection, target selection and CLEM registration make it the best target selection workflow for PACEtomo on any kind of lamella sample. - Easier installation and configuration Any feedback welcome!

Some translating but stalled bacterial ribosomes. Tandem purification using RAPPL. Ready for cryoEM...one can almost see beads on the string

Pac-Man in real life! #mitophagy

Looking thru old lab EM and found this monstrosity…. They’re almost like train tracks, anyone have an idea what’s going on here? Never seen this before! This is in a mutant background also. Martin Picard

#cryoEM open heart surgery.... They are about to hook me up for a blood infusion.... The Thermo Fisher engineers have got the sterile syringes ready! 🤣

I’m experiencing repeated errors during tomography data acquisition. Any suggestions on how to resolve this issue? Thanks in advance.

Reprocessing some old #cryoEM data from my time in Andres Leschziner lab. Gonna use this to prove to my students that I used to be awesome in the lab! 2.7 Å from 550 micros, hand-selected in Leginon and collected in super-res (gasp!) on a Talos with a K2. Ancient by today's standards

Windows has come a long way! Relion 5.0 compiled fine in the WSL-2 and now runs natively in Windows.... Very cool! #cryoem

Good day ISMB_London brining back the FEI Tecnai T10 & T12 electron microscopes 🔬with a O-ring fixes, filament change, and replacement of the high tension tank (easy lift) with Thermo Fisher engineers. #cryoem.

Running an early 2000s version of #xmipp and #chimera on a 1996 Silicon Graphics Indigo² workstation. These pieces of software have stablished the foundations for many modern #CryoEM image processing packages.

Received some nice hardware from SubAngstrom to increase #cryoEM ❄️🔬storage for our users Birkbeck, University of London - ISMB_London. 4 grids per grid box 📦, 12 grid boxes per puck 🏒, 10 pucks per cane. That is 120 grid boxes or 480 grids in a cane - should keep the Krios busy. Hopefully

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it! guide.cryosparc.com/processing-dat…

The coolest thing I've said today was "I'm gonna charge my grid" 🤓. I'm officially in the cryoEM world. 🥳 It is never too late. Keep learning. Keep doing hard things.