Haha. One sec…#dermpathjc

Still getting used to Twitter again… #dermpathjc

For those interested, I can give a little background on this paper…..#dermpathjc

GLI-1 rearrangements have been known for a little while, but in soft tissue and head/neck literature. Not so much derm…..#dermpathjc

The details of back story may not be 100% accurate, but I believe Konstantinos Linos accumulated 2 cases in collab with other authors…. #dermpathjc

He got Julia Bridge involved, who works w me, as she is true FISH expert. #dermpathjc

Julia shares all her weird derm consults w me, which I am thank for. Something was id’d on NGS, and then FISH performed/requested to see if there was GLI-1 amp ….#dermpathjc

It has peculiar histology, right? Particularly that “pericyte” pattern where it hugs vessels…..#dermpathjc

While these 2 cases were being studied and written up, I got a routine one from a derm in TX that looked very similar…I mean, what are the odds?!?!? #dermpathjc

To make long story short, all 3 had amplified GLI-1. These tumors can have rearrangements too…..#dermpathjc

Yes. Variable biology tho, it seems. It some soft tissue tumors, there is variability in biology based on whether there is rearrangement vs amplification, but not enough is know about these

Yes! That is what caught my eye. It looked almost sinusoidal as the cells filled stroma around the vessels, but not true “vascular” tumor. I would lump it w the “perivascular” family of tumors

Idk!! I wonder that too. I suspect pretty rare but not sure. #dermpathjc

The other really cool think Bridge did was map the areas of amplification for the 3 tumors using different probes to the chromosome #dermpathjc

She found that some included MDM2, CDK4, +/- STAT6, and it seemed to correlate w the IHC results #dermpathjc

Good question actually, and not 100% sure what best panel is. CD56 strong. The MDM2, CDK4, STAT6 are good ones but only if those regions are also amplified (may be neg for tumors w rearrangements, but not sure) #dermpathjc

I do start to wonder that tumors which are not readily recognizable will soon be triaged into “expression panels” (IHC) or molecular panels (NGS), but possibly not both?!? Thoughts? #dermpathjc

I’m working on one right now that I don’t recognize, and have done extensive IHC but it “looks” like something that likely will have a translocation or other consistent molecular abnormality. Should have gone straight to NGS #dermpathjc

I’m checking out the rest of ur fridge. What else? #dermpathjc

Haha. Nobody fact checked me! It was great! Haha #dermpathjc