$180 million DNA ‘barcode’ project aims to discover 2 million new species. bit.ly/2KLCm9I

Bacteria to DNA in less than 1 hr! No post-extraction size selection required due to optimal molecular ratio of long to short DNA fragments. At Q11 N50~50Kb, Yield 5+Gb, and 2,000+ reads above 100Kb using a MIN106D flow cell LSK109 Oxford Nanopore . (Predicted coverage at Q7 >3700x)

Honored that our paper got featured by Nanopore daily digest! Thx! "Tracking of antibiotic resistance transfer and rapid plasmid evolution in a hospital setting by Nanopore sequencing" Oxford Nanopore Mattia Bosio Stephan Ossowski nanoporetech.com/resource-centr…

An E.Coli Fire Monkey extract ran on LSK109 enabled a complete assembly with ~1000x coverage of the chromosome (Flye, Q7) and recovery of a ~130kb plasmid (Q7, ~1000x coverage) using LSK109 Oxford Nanopore

Long reads less reliant on the cold-chain! DNA/RNA Shield Zymo Research is compatible with Fire Monkey. 20+Gb and 6,000+ reads above 100kb with 968 MIN106D pores Oxford Nanopore active after 48hrs of sequencing.

RevoluGen signs Merck as Global distribution partner for Fire Monkey!

'Long Shot' LSK109 pushes Fire Monkey's performance on a re-used MIN106D flow cell Oxford Nanopore (which yielded 22 Gb on 1st run) after 6hrs. N50 increased by ~17% and 100kb+ reads were more than doubled. Optimisation is now under-way!

The Near East history could be full of transient pulses of genetic mixing that disappeared after few generations Medieval period: cell.com/ajhg/fulltext/… Iron Age: advances.sciencemag.org/content/5/7/ea…

20mins of LSv2 promotes Fire Monkey long DNA access through Femto Pulse capillaries Agilent Technologies and results in >10% of the yield above 100kb Oxford Nanopore. More to follow!

Archaeologists are, for the first time, discovering the probable origins of ancient Greece. bit.ly/2JtGnyo

Improved Long Shot (LSv3/LSK109) protocol on Fire Monkey extract is generating a long tail on MIN106D Oxford Nanopore. MinKNOW estimates top read of 575kb after 1hr of sequencing!

Fire Flower size selects 200microLt of extracted DNA. 15mins of spins increase % of molecules above 30kb and ~double most long read metrics. 48hrs runs on LSK109/MinION Oxford Nanopore

Emma Waters showing how long read sequencing aids operon rearrangements in salmonella at #genomescience2019

15mins of Fire Flower treatment on a very challenging DNA input sample more than doubles average and N50. Long read numbers remain the same. 48hrs runs on LSK109/MinION Oxford Nanopore

....after 1 polishing step. 50x initial coverage with fragments longer than 122kb!

Input average at 115kb (Femto Pulse). Pores at start: 1273, after 29hrs: 52, after nuclease flush: 878. So more nuclease flushes for us from now on.

Compared to same sample input for Fraction B only, at 48hrs, no nuclease flush we get (at Q11) ~3Gb, N50: ~53KB and ~2000 100KB+ reads.

Too eager to run R10 over W/E so no nuclease flush, plus maybe added a bit too much DNA, but FM-R10 work well together.

Today's @Nanopore #Nanoporn. They say that the Fire Monkey was irrepreseable! #FireMonkeyMagic #longreads