Luke Lavis Merz Edward Marcotte J. Am. Chem. Soc. more JF spectra than you can shake a stick at. (2P coming soon) thanks Luke Lavis! fpbase.org/spectra/?s=637…

Databases are hard... and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone! Here's an example of what not to do if you find something needing correction 😂

📣 Apply for an award from Addgene to attend a conference! We're accepting applications until April 30 ☑️ Undergrad, grad student, postdoc, research tech ☑️ Working w/ fluorescent proteins ☑️ Planning to attend a conference, virtual, hybrid, or in-person hubs.ly/H0LbWNd0

a reminder on the difficulty in comparing fluorescence photobleaching measurements across studies (and even across probes on the same scope) fpbase.org/bleaching/

Dear imaging companies, Please tell your customers (or make available on request) the *actual* spectra for all components (LEDs, filters, etc) in their light path. just saying "460-490nm" is not sufficient. It's amazing how many scientists can't get this info from their reps 😞

This is beautiful and makes me want to rewrite FPbase using it 😍

Just discovered the interactive chart of FPbase.com. Wow, what an amazing tool to compare different properties across a wide range of fluorescent proteins. Great work Talley Lambert 🙂 fpbase.org/chart/

Christoph Spahn Talley Lambert FPbase is amazing! I created scope configurations for University of Arizona Cancer Center core instruments, and link to it with a little QR code posted next to the scope! And made a nice lab exercise for my microscopy class. iScopeCalc rocks too. Keep ‘em coming, Talley Lambert!

🥁 Delighted to announce that our FPCountR paper is now on bioRxiv! In this paper we describe the development of a method to allow absolute fluorescent protein quantification using microplate readers for synthetic circuit characterisation. biorxiv.org/content/10.110… 🧵

Always nice to see features put to use! Eszter Csibra and Prof Guy-Bart Stan use the FPbase API (among other things) in a method for the calibration of fluorescence readings on microplate readers; for quantification of FP concentration biorxiv.org/content/10.110…

anyone out there routinely express FPs in Chlamydomonas? any modern suggestions for SNR? (presumably red, but any expression gotchas?)

this is genius: University of Helsinki BioImaging Unit utilizes FPbase to allow users test their fluorophores fpbase.org/microscope/u4g… cc Daniel Waiger

Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing Mayank Sharma!

Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at Nature Methods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s4159…

Mutagenesis of #FbFP #LOV domain: 🟠 22 variants with 486-512 nm emission maxima 🟠 three-channel imaging via spectral unmixing 🟠 two-channel fluorescence lifetime unmixing doi.org/10.1101/2022.1… Congratulations, Ivan Gushchin, Anna Yudenko, and all the authors! FPbase

recently hit 2,000,000 sessions on FPbase ! 📈

This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from FPbase.

Hi Fluorescence friends! Please follow future updates on Bluesky: bsky.app/profile/fpbase…