Lale Ertuglu, MD Guto Montezano Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Hypertension Meena Madhur David G Harrison Did see differences in pulse pressure as a crude estimate of arterial stiffness? #hyphip Guto Montezano Hypertension Lale Ertuglu, MD Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A.

Guto Montezano Ashley Pitzer Lale Ertuglu, MD Hypertension A14 salt-sensitive people have super sensitive and high expression of SGK1. Our data indicates not through the MR. It’s the GR. Aldo does not correlate with salt-sensitive hypertension #HYPHIP Hypertension

Lale Ertuglu, MD Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Hypertension Meena Madhur David G Harrison It would be interesting to know beyond vascular dysfunction, maybe looking at non-invasive measurements in the subjects going on the salt-sensitivity protocol. Not sure if all makes sense though. #HYPHIP Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD Hypertension

Q15 Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD With the rise of K as a Na substitute, what would you expect if you used K-enriched salt? #HYPHIP Hypertension

Guto Montezano Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Hypertension A13 2/2 While we could not assess vascular function in the current study, previously Meena Madhur David G Harrison found that T-cell activation and IL-17A production lead to vascular dysfunction by altering vascular reactivity and increasing vascular superoxide Hypertension #HYPHIP

Guto Montezano Ashley Pitzer Lale Ertuglu, MD Hypertension A13. Yes indeed. We find vascular dysfunction plays a role. Endothelial cells produce VCAM, ICAM that attract immune cells, which then gain access to tissues #HYPHIP Hypertension

A11 #HYPHIP

A11 continuation #HYPHIP

A11 2/2 #HYPHIP

Q14 Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD In your data, you didn't see a role for aldosterone. Do you know if in isolevuglandin-activated APCs, MR activation is increased? And What about cortisol? #HYPHIP Hypertension

Guto Montezano Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Hypertension A13 1/2 This is a great question. Dr. Laffer and colleagues previously showed that salt sensitivity is associated with increased vascular resistance, suggesting that vascular dysfunction plays a key role in salt sensitivity. Hypertension #HYPHIP

Q13 Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD You have seen an association between IsoLv activation of APCs and pro-inflammatory cytokines in salt-sensitivity. Are these changes are associated with changes in vascular function or structure in your cohort? #HYPHIP Hypertension

Tanja Dudenbostel, MD, FASH, FAHA Guto Montezano Hypertension Ashley Pitzer More in women even in pre-menopause but amplified by menopause

Tanja Dudenbostel, MD, FASH, FAHA Guto Montezano Hypertension Ashley Pitzer We are not yet powered to answer this for immune mechanisms - it is ongoing but all large clinical trials that have rigorously phenotyped salt-sensitivity find it mainly in women

Migdalia Buchan Francisco Rios Ashley Pitzer Lale Ertuglu, MD Hypertension Guto Montezano To clarify, in our paper we think EETs regulating APC ENaC are coming from the kidney. Not sure what specific cell type in the kidney but endothelial cells could be a source #HYPHIP Hypertension

Lale Ertuglu, MD Guto Montezano Ashley Pitzer Hypertension Our findings that blood cells are salt-sensitive are game changing in terms of coming up with a feasible diagnosis #HYPHIP Hypertension

Guto Montezano Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Hypertension A9 3/3 We excluded patients who were on medications that modulate the immune system or blood pressure. For patients on anti-hypertensive medications, all antihypertensives were discontinued for at least 2 weeks before the study. Hypertension #HYPHIP

A9 1/3 #HYPHIP

Q10. Do you have any suggestion about what T cell population/cytokines would be involved in such mechanisms?
Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD #HYPHIP
Hypertension

Q10. What your suggestion about which T cell population would be involved in this mechanism?
Ashley Pitzer Annet Kirabo D.V.M., M.Sc., Ph.D., F.A.H.A. Lale Ertuglu, MD